is an emerging multidrug-resistant yeast that has been systematically incorrectly identified by phenotypic methods in clinical microbiology laboratories. The Vitek 2 automated identification system (bioMérieux) recently included in its database (version 8.01). We evaluated the performance of the Vitek 2 YST ID card to identify and related species. A panel of 44 isolates of species (, = 35; , = 5; , = 4) were tested by three different hospital-based microbiology laboratories. Among 35 isolates of , Vitek 2 yielded correct identification in an average of 52% of tested samples. Low-discrimination (LD) results with an inability to distinguish between , , and were obtained in an average of 27% of samples. Incorrect identification results were obtained in an average of 21% of samples, the majority (91%) of which were reported as and the remaining 9% of which were reported as The proportion of correct identification was not statistically different across different centers ( = 0.78). Stratification by genetic clades demonstrated that 100% ( = 8) of the strains of the South American clade were correctly identified compared to 7% ( = 10) and 0% ( = 4) from the African and East Asian clades, respectively. None of the non- strains ( = 9) were incorrectly identified as Our results show that the Vitek 2 (version 8.01) yeast identification system has a limited ability to correctly identify These data suggest that an identification result for should warrant further testing to rule out The overall performance of the Vitek 2 seems to differ according to genetic clade, with the South American isolates yielding the most accurate results.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812989 | PMC |
http://dx.doi.org/10.1128/JCM.00884-19 | DOI Listing |
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