AI Article Synopsis
- Glaesserella parasuis is a significant pathogen in pigs, causing high illness and death rates among naive populations, making quick detection essential for managing outbreaks.
- The newly developed RPA-LF-GPS method can accurately detect G. parasuis at a low level of 100 CFU and is just as effective using boiled synovial fluid samples as it is with traditional DNA extraction methods.
- This point-of-care testing method completes the testing process in just one hour and shows comparable sensitivity to PCR testing, making it a practical option for diagnosing G. parasuis infections.
Article Abstract
Background: Glaesserella parasuis (G. parasuis) is an influential pathogen of the pig, which induces high morbidity and mortality in naive pig populations in the pig industry. Accurate and rapid detection of the agent is important for disease control. In this study, a simple recombinase polymerase amplification (RPA) with a Lateral flow (LF) strip (RPA-LF-GPS) was developed to detect G. parasuis.
Results: The RPA-LF-GPS can specifically detect G. parasuis a limit of 100 CFU from other common related pathogens causing arthritis in the pig. The RPA-LF-GPS assay can use boiled synovial fluid samples as a template with the same sensitivity as other DNA extraction methods. In the detection of clinic positive synovial fluid sample, RPA-LF-GPS is equally sensitive (98.1%) compared with that of PCR (90.4%) (P > 0.05). The whole procedure of the RPA-LF-GPS assay could be finished in 1 hour without professional equipment.
Conclusions: RPA-LF-GPS assay is a rapid and simple method for point-of-care diagnostic testing for G. parasuis infection.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694577 | PMC |
| http://dx.doi.org/10.1186/s12917-019-2039-x | DOI Listing |
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