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Expression of Osteoblast-Specific Factor 2 (OSF-2, Periostin) Is Associated with Drug Resistance in Ovarian Cancer Cell Lines. | LitMetric

AI Article Synopsis

  • Ovarian cancer treatment is challenging due to cancer cells developing resistance to drugs, with OSF-2 (Osteoblast-Specific Factor 2) being linked to this problem.
  • The study examined OSF-2 expression in drug-sensitive (W1) and drug-resistant (W1DR for doxorubicin and W1MR for methotrexate) ovarian cancer cell lines through various analyses.
  • Results indicated that drug-resistant cells had higher OSF-2 mRNA and protein levels, suggesting that OSF-2 may play a role in the resistance to doxorubicin and methotrexate treatments in ovarian cancer.

Article Abstract

One of the main obstacles to the effective treatment of ovarian cancer patients continues to be the drug resistance of cancer cells. Osteoblast-Specific Factor 2 (OSF-2, Periostin) is a secreted extracellular matrix protein (ECM) expressed in fibroblasts during bone and teeth development. Expression of OSF-2 has been also related to the progression and drug resistance of different tumors. The present study investigated the role of OSF-2 by evaluating its expression in the primary serous ovarian cancer cell line, sensitive (W1) and resistant to doxorubicin (DOX) (W1DR) and methotrexate (MTX) (W1MR). The transcript (real-time PCR analysis), protein expression in cell lysates and cell culture medium (western blot), and expression of the OSF-2 protein in cell lines (immunofluorescence) were investigated in this study. Increased expression of mRNA was observed in drug-resistant cells and followed by increased protein expression in cell culture media of drug-resistant cell lines. A subpopulation of ALDH1A1-positive cells was noted for W1DR and W1MR cell lines; however, no direct co-expression with OSF-2 was demonstrated. Both drugs induced expression after a short period of exposure of the drug-sensitive cell line to DOX and MTX. The obtained results indicate that OSF-2 expression might be associated with the development of DOX and MTX resistance in the primary serous W1 ovarian cancer cell line.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719218PMC
http://dx.doi.org/10.3390/ijms20163927DOI Listing

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