AI Article Synopsis
- - The mitochondrial unfolded protein response (UPR) triggers increased production of the chaperone HSP70 by enhancing the transcription of the hsp-6 gene.
- - A study identified 171 genes in C. elegans that, when silenced, lead to increased expression of hsp-6 and are involved in mitochondrial processes, although not all mitochondrial functions were covered.
- - Silencing these genes reduces mitochondrial membrane potential and protein import, activating UPR through ATFS-1, which acts as a sensor for signals indicating compromised mitochondrial protein import.
Article Abstract
The induction of the mitochondrial unfolded protein response (UPR) results in increased transcription of the gene encoding the mitochondrial chaperone HSP70. We systematically screened the C. elegans genome and identified 171 genes that, when knocked down, induce the expression of an hsp-6 HSP70 reporter and encode mitochondrial proteins. These genes represent many, but not all, mitochondrial processes (e.g., mitochondrial calcium homeostasis and mitophagy are not represented). Knockdown of these genes leads to reduced mitochondrial membrane potential and, hence, decreased protein import into mitochondria. In addition, it induces UPR in a manner that is dependent on ATFS-1 but that is not antagonized by the kinase GCN-2. We propose that compromised mitochondrial protein import signals the induction of UPR and that the mitochondrial targeting sequence of ATFS-1 functions as a sensor for this signal.
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| http://dx.doi.org/10.1016/j.celrep.2019.07.049 | DOI Listing |
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