Detection of in piglet processing fluids.

Processing fluid (PF) is a sample type composed of fluids obtained from testicles and tails as the product of piglet processing. is a bacterium that colonises the respiratory tract of pigs and has rarely been detected in tissues outside the respiratory system. No data exist in the literature regarding detection of in PF or its use for herd monitoring of this pathogen. The main goal of this study was to evaluate the feasibility of detecting in PF. Testicles and tails of 21 conveniently selected litters from a commercial sow farm were collected, by litter, and tested for by real time-PCR. Daily aggregated processing tissues were collected for a two-month period to assess the detection of in PF. The comparison in the percentage of positive samples in fluids from testicles (38 per cent, 8/21) or tails (4.8 per cent, 1/21) was significantly different (P=0.023). The percentage of daily aggregated PF with cycle threshold values up to 37 was 52.9 per cent (9/17) and 26.7 per cent (4/15) for December and January, respectively. Overall, these data show detection of in PF for the first time and points at the potential use of this sample for monitoring of this bacterium in breeding farms.