In order to assess the metastatic potential of residual hepatocellular carcinoma (HCC) following insufficient radiofrequency ablation (RFA) and to improve the current animal model, an insufficient RFA orthotopic nude mouse model of HCC was developed in the present study. A human HCC orthotopic nude mouse model was established using HCCLM3 cells, which has a high metastatic potential, labeled with green fluorescent protein. A total of 12 nude mice within the RFA group received insufficient RFA and 12 mice in the control group received RFA needle electrode puncture of the tumor without ablation, 3 weeks after implantation. To investigate tumor growth and metastasis, 4 weeks after RFA, six mice in each group were sacrificed and the remaining mice in each group were maintained until death to evaluate their life span. No mice died following insufficient RFA and the success rate was 100%. Compared with the control group, the intrahepatic and lung metastasis rates were higher in the RFA group, despite the mice having smaller tumor volumes and longer survival times. Lung and intrahepatic metastasis rates in the insufficient RFA group were 100% (6/6) and 66.67% (4/6), respectively, compared with 33.33% (2/6) and 0% (0/6), respectively, in the control group. As part of the study, a safe and reliable method to establish an insufficient RFA orthotopic nude mouse model was developed. The present study revealed that residual cancer following insufficient RFA had exhibited increased invasiveness and metastatic potential.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6676705 | PMC |
| http://dx.doi.org/10.3892/ol.2019.10552 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Retrospective Study of Outcomes in a Rural Pediatric Population Treated for Venous Insufficiency.
J Pediatr Surg
December 2024
Valley Vein Health Center, 840 Delbon Ave, Turlock, CA 95382, USA; University of Central Florida, College of Medicine, 6850 Lake Nona Blvd, Orlando, FL 32827, USA.
Background: Endovascular radiofrequency ablation (RFA) and Endovascular Laser Ablation (EVLA) are minimally invasive methods to safely treat symptomatic varicose veins in pediatric patients. This research aimed to review the management of pediatric patients with venous insufficiency, evaluate the outcome, and determine the efficacy, convenience, and safety of ablation procedures in an outpatient setting.
Methods: A retrospective chart review of all patients seen at six locations from 2013 to 2024 was completed.
Feasibility and Safety of Flush Endovenous Thermal Ablation of the Great Saphenous Vein with Consecutive Foam Sclerotherapy of Saphenofemoral Junction Tributaries: A Single-Center Experience.
J Clin Med
November 2024
Department of Vascular Surgery, Jun's Vascular Clinic, Busan 47256, Republic of Korea.
Blood flow from the saphenofemoral junction(SFJ) tributaries may cause recurrence of varicose veins. Flush occlusion is defined as the total occlusion of the great saphenous vein(GSV) right to the saphenofemoral junction. The purpose of this study was to evaluate the efficacy and safety of flush endovenous thermal ablation with saphenofemoral junction tributary occlusion.
View Article and Find Full Text PDFResonance frequency analysis of dental implants in patients with vitamin D deficiency.
Clin Oral Investig
December 2024
Department of Periodontology, Faculty of Dentistry, Inonu University, 44280, Malatya, Turkey.
Article Synopsis
- - The study investigated how different levels of vitamin D affect the stability of dental implants, separating patients into four groups based on their vitamin D levels and whether they were taking supplements.
- - Results showed that patients with sufficient vitamin D (Group D) had significantly higher implant stability than those with deficiency or insufficiency, particularly at the baseline measurement.
- - The conclusion emphasized that higher vitamin D levels are linked to better implant stability, suggesting that supplementation is critical for patients with low vitamin D when undergoing dental implants.
The long-term efficacy of radiofrequency ablation for varicose veins in patients with iliac vein compression.
Asian J Surg
November 2024
Department of Vascular Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing, China. Electronic address:
Article Synopsis
- - This study investigated the long-term effectiveness of radiofrequency ablation (RFA) for treating varicose veins (VVs) in patients with grade II iliac vein compression (IVC), involving 511 patients over three years.
- - Results showed significant improvements in both venous clinical severity scores (VCSS) and chronic venous insufficiency questionnaire (CIVIQ) scores for both the VVs-only group and the VVs + IVC group, with no major differences in outcomes between the two groups.
- - The findings suggest that RFA is a strong and lasting treatment option for varicose veins in patients with grade II IVC, providing a viable alternative to more invasive stent procedures.
Comparative study on the effectiveness and safety of simultaneous and staged bilateral saphenous vein radiofrequency ablation.
J Vasc Surg Venous Lymphat Disord
November 2024
Department of Cardiovascular Surgery, Antalya Education and Training Hospital, University of Health Science, Antalya, Turkey.
Objective: Chronic venous insufficiency (CVI) is a common condition affecting more than one-half of the general population, with approximately 20% of cases being bilateral. The high prevalence, diagnosis, and treatment costs, along with the negative impact on patients' quality of life, underscore its significance. This study evaluates the clinical outcomes, quality-of-life effects, and postoperative complications of simultaneous and staged bilateral radiofrequency ablation (RFA) in patients with bilateral CVI in the great saphenous vein at Clinical, Etiological, Anatomical, Pathophysiological stages 2 to 4.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!