Purpose: SeptiCyte LAB measures the expression of four host-response RNAs in peripheral blood to distinguish sepsis from sterile inflammation. This study evaluates whether sequential monitoring of this assay has diagnostic utility in patients after esophageal surgery.
Materials And Methods: Patients who developed a complication within 30 days following esophageal surgery and a random sample of 100 patients having an uncomplicated course. SeptiCyte LAB scores (ranging 0-10 reflecting increasing likelihood of infection) were compared to post-hoc physician adjudication of infection likelihood.
Results: Among 370 esophagectomy patients, 120 (32%) subjects developed a complication requiring ICU (re)admission, 63 (53%) of whom could be analyzed. Immediate postoperative SeptiCyte LAB scores were highly variable, yet similar for patients having a complicated and uncomplicated postoperative course (median score of 2.4 (IQR 1.6-3.3) versus 2.2 (IQR 1.3-3), respectively). In a direct comparison of patients developing a confirmed infectious (n = 34) and non-infectious complication (n = 12), addition of SeptiCyte LAB to CRP improved diagnostic discrimination of infectious complications (AUC 0.88 (95%CI 0.77-0.99)) compared to CRP alone (AUC 0.76 (95%CI 0.61-0.91); p = .04).
Conclusions: Sequential measurement of SeptiCyte LAB may have diagnostic value in the monitoring of surgical patients at high risk of postoperative infection, but its clinical performance in this setting needs to be validated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jcrc.2019.07.020 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transcriptional pathways of terminal differentiation in high- and low-density blood granulocytes in sepsis.
J Inflamm (Lond)
October 2024
Department of Anesthesiology, Surgical Intensive Care Medicine and Pain Medicine, Mannheim Institute of Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Article Synopsis
- Trauma and infections trigger emergency granulopoiesis, which leads to an increase of immature granulocytes in the blood during severe conditions like sepsis, but their effectiveness as biomarkers is limited due to mixed disease severity responses.
- * A study was conducted comparing sepsis patients to those with SIRS, using techniques like flow cytometry and gene expression assays to analyze granulocyte populations and their developmental stages.
- * Results showed that immature granulocyte precursor counts were higher in sepsis but didn't correlate with disease severity, and that low-density granulocytes had a significantly greater number of precursors compared to high-density granulocytes.
Putative mRNA Biomarkers for the Eradication of Infection in an Equine Experimental Model of Septic Arthritis.
Vet Sci
July 2024
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Septic arthritis (SA) in horses has long-term health implications. The success of its resolution hinges on the implementation of early, aggressive treatment, which is often sustained over a prolonged period. Common diagnostic methods do not allow for the reliable detection of the eradication of joint infection.
View Article and Find Full Text PDFDiagnosing sepsis in the ICU: Comparison of a gene expression signature to pre-existing biomarkers.
J Crit Care
August 2023
Intensive Care Services, Royal Brisbane and Women's Hospital, Herston, Queensland, Australia; Jaimeson Trauma Institute, Royal Brisbane and Women's Hospital, Herston, Queensland, Australia; Nimes University Hospital, University of Montpellier, Nimes, France.
Purpose: We aimed to identify a gene signature that discriminates between sepsis and aseptic inflammation in patients administered antibiotics in the intensive care unit and compare it to commonly utilised sepsis biomarkers.
Methods: 91 patients commenced on antibiotics were retrospectively diagnosed as having: (i) blood culture positive sepsis; (ii) blood culture negative sepsis; or (iii) aseptic inflammation. Bloods were collected after <24 h of antibiotic commencement for both gene expression sequencing analysis and measurement of previously identified biomarkers.
A pilot study of a novel molecular host response assay to diagnose infection in patients after high-risk gastro-intestinal surgery.
J Crit Care
December 2019
Department of Intensive Care, University Medical Center Utrecht, Utrecht, the Netherlands.
Purpose: SeptiCyte LAB measures the expression of four host-response RNAs in peripheral blood to distinguish sepsis from sterile inflammation. This study evaluates whether sequential monitoring of this assay has diagnostic utility in patients after esophageal surgery.
Materials And Methods: Patients who developed a complication within 30 days following esophageal surgery and a random sample of 100 patients having an uncomplicated course.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!