Azadirachtin, a botanical insecticide with high potential, has been widely used in pest control. Azadirachtin has shown strong biological activity against Bactrocera dorsalis in toxicological reports, but its mechanism remains unclear. This study finds that azadirachtin A inhibits the growth and development of Bactrocera dorsalis larvae. The larval weights and body sizes of the azadirachtin-treated group were significantly less than those of the control group in a concentration-dependent manner. Further, pathological sections revealed that azadirachtin destroyed the midgut cell structure and intestinal walls, while TUNEL staining showed that azadirachtin could induce apoptosis of midgut cells, and Western blot analysis indicated that Bcl-XL expression was inhibited and cytochrome c (CytC) released into the cytoplasm. The results also imply azadirachtin-induced structural alterations in the Bactrocera dorsalis larvae midgut by activation of apoptosis. RNA-seq analysis of midgut cells found that 482 and 708 unique genes were upregulated and downregulated, respectively. These differentially expressed genes (DEGs) were enriched in apoptotic and lysosomal signaling pathways and included 26 genes of the cathepsin family. qRT-PCR verified the expression patterns of some DEGs, indicating that Cathepsin F was upregulated by 278.47-fold and that Cathepsin L and Cathepsin D were upregulated by 28.06- and 8.97-fold, respectively. Finally, association analysis between DEGs and DEMs (differentially expressed metabolites) revealed that azadirachtin significantly reduced the digestion and absorption of carbohydrates, proteins, fats, vitamins and minerals in the midgut. In conclusion, azadirachtin induces the release of cathepsin from lysosomes, causing apoptosis in the midgut. Ultimately, this leads to reduced digestion and absorption of nutrient metabolites in the midgut and inhibition of the growth and development of Bactrocera dorsalis larvae.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ecoenv.2019.109512 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The genus Psyttalia Walker is recorded for the first time from Vietnam with seven species described and fully illustrated as new for science, viz. Psyttalia antenervis Long, sp. nov.
View Article and Find Full Text PDFLife history traits of the target pest and transmission routes of the biocide are critical for the success of the boosted Sterile Insect Technique.
Curr Res Insect Sci
November 2024
CIRAD, UMR CBGP, F-34398 Montpellier, France.
The sterile insect technique (SIT) is an environmentally friendly pest control strategy that consists of inundative releases of mass-reared sterilized males over defined areas, where they mate with wild females, resulting in no offspring and a declining pest population. The technique has effectively managed many crop pests and vector-borne diseases worldwide. A new approach, called boosted SIT, has been proposed to gain efficiency.
View Article and Find Full Text PDFMolecular module for glucose production influences sex pheromone synthesis in Bactrocera dorsalis.
Cell Rep
December 2024
Department of Entomology, South China Agricultural University, Guangzhou 510640, China. Electronic address:
Article Synopsis
- Some insects, like male Bactrocera dorsalis, rely on intestinal microbes for producing sex pheromones to communicate with each other.
- This study shows that these microbes produce sex pheromones in the evening and that glucose levels play a crucial role in this production.
- The research identifies a molecular mechanism involving genes that regulate glucose, linking how the host insect can control pheromone synthesis through its intestinal microbes.
Bdyof is a Y-chromosome-specific gene required for male development in Bactrocera dorsalis.
Pest Manag Sci
November 2024
National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Background: In many organisms, the Y chromosome contains important genes associated with sex determination and male reproductive development. However, there have been few studies of Y-chromosome-specific genes in non-model species due to the incomplete information of Y chromosome genome and difficulty in sequencing. Here, we screened 90 candidate Y-specific sequences in a constructed transcriptome assembly library by using the chromosome quotient method, among which 11 were unreported sequences associated with male reproductive development, including Bactrocera dorsalis Y-specific Oligozoospermia factor (Bdyof) with the highest expression in the testis.
View Article and Find Full Text PDFTemperature-Dependent Pupation Depth in the Oriental Fruit Fly and Its Implications for Biological Control.
Insects
November 2024
Department of Entomology, National Taiwan University, Taipei 10617, Taiwan.
The oriental fruit fly, (Hendel) (Diptera: Tephritidae), is a notable agricultural pest that undergoes pupation in the soil. Mortality risk from predation and parasitism decreases as the depth of the pupal location increases from the ground surface, with a one-centimetre increase in depth causing a significant change. Soil properties, such as moisture and hardness, influence pupation depth, but the effect of temperature has not been fully tested.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!