Zika virus (ZIKV) serological diagnostics are compromised in areas where dengue viruses (DENV) co-circulate because of their high levels of protein sequence homology. Here, we describe the characterization of a Zika blockade-of-binding ELISA (Zika BOB) and a Zika microneutralization assay (Zika MN) for the detection of ZIKV nonstructural protein 1 (NS1)-specific antibodies and ZIKV neutralizing antibodies, respectively. Zika BOB and Zika MN cutoffs were established as 10 and 100 endpoint titers, respectively, using samples collected pre- and post-virologically confirmed ZIKV infection from subjects living in DENV-endemic areas. Specificity of the assays was equally high, whereas sensitivity of Zika BOB was lower than that of Zika MN, especially in samples collected > 6 months post-infection. Immunosurveillance analysis, using combined results from both Zika BOB and Zika MN, carried out also in DENV-endemic regions in Colombia, Honduras, Mexico, and Puerto Rico before (2013-2014) and after (2017-2018) ZIKV introduction in the Americas suggests unapparent ZIKV seroprevalence rates ranged from 25% to 80% over the specified period of time in the regions investigated.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726926PMC
http://dx.doi.org/10.4269/ajtmh.19-0270DOI Listing

Publication Analysis

Top Keywords

zika bob
16
zika
12
bob zika
12
blockade-of-binding elisa
8
microneutralization assay
8
zika virus
8
samples collected
8
zikv
6
elisa microneutralization
4
assay evaluate
4

Similar Publications

Article Synopsis
  • The Zika virus has caused autochthonous transmission in 87 countries since 2015, posing risks like Guillain-Barré syndrome and complications in pregnancy, underscoring an urgent need for effective vaccines and enhanced understanding of immunity against ZIKV.
  • Researchers developed a new diagnostic test called blockade-of-binding (BOB) ELISA, using specific monoclonal antibodies to differentiate ZIKV from other flaviviruses, and found it performed better than traditional tests in validating ZIKV infections and assessing vaccine responses.
  • The BOB assays demonstrated high sensitivity and specificity in detecting antibodies from recent and past ZIKV infections, indicating potential for broader applications in monitoring ZIKV immunity and evaluating vaccine efficacy.
View Article and Find Full Text PDF

Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as a global health threat. The rise in ZIKV infections has driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for ZIKV infection can considerably impact pregnant women, especially during the first trimester.

View Article and Find Full Text PDF
Article Synopsis
  • The study focused on pediatric dengue and Zika virus surveillance in rural Guatemala during and after the 2015-2016 Zika virus pandemic.
  • The results showed a slight increase in dengue seroprevalence, while Zika seroprevalence rose significantly from 6% to 34% over three months.
  • Key risk factors for Zika seropositivity were older age and the literacy of primary caregivers, highlighting the potential for rapid active surveillance as an effective method in resource-limited areas to track these viruses.
View Article and Find Full Text PDF

Background: The prevalence of developmental alterations associated with in-utero Zika virus (ZIKV) exposure in children is not well understood. Furthermore, estimation of the Population Attributable Fraction (PAF) of developmental alterations attributed to ZIKV has not been performed due to lack of population-based cohorts with data on symptomatic and asymptomatic ZIKV exposures and an appropriate control group. The aim of this study was to characterize neurodevelopmental outcomes of children at 11 to 32 months of age with intrauterine ZIKV exposure and estimate the PAF of alterations secondary to ZIKV exposure.

View Article and Find Full Text PDF

Background: Zika virus caused thousands of congenital anomalies during a recent epidemic. Because Zika emerged in areas endemic for dengue and these related flaviviruses elicit cross-reactive antibodies, it is challenging to serologically monitor pregnant women for Zika infection.

Methods: A prospective cohort of 253 pregnant women was established in León, Nicaragua.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!