Carotenoids have good biological activity in antioxidant, anti-aging and scavenging harmful free radicals. In this study, we screened a strain that produced carotenoids, and selected a stress condition which significantly improved carotenoids content. The strain was identified as PR106. Active oxygen generator TiO was the most significant factor to the carotenoids content of the . The content of carotenoids was 54.45 mg/g at 500 mg/L TiO, which was about 1.25 times of the control and the proportion of carotenoids also changed from 1:9:16 to 1:8.5:12. Further, we determined the reactive oxygen species (ROS) in YEPD medium and , found that the ROS (HO, O, and HO•) was significantly increased at 500 mg/L TiO in YEPD medium compared with the control, but increased in under 1000 mg/L TiO treated. These results suggested that the increase in carotenoids was related to ROS in .
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