A label-free quantification method for measuring graphene oxide in biological samples.

Characterization of carbonaceous nanomaterials (CNMs) exposure is a key step and of great importance towards a better understanding of their toxicity and underlying mechanisms. However, it has been bottlenecked for lack of valid methods capable of quantifying cell-associated CNMs. Here, we developed a new economical and convenient method based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) that could accumulate graphene oxide (GO) at the interface between the loading well and the gel. The sharp black band formed there can be digitalized and the intensity quantified, which was proportional to the amount of GO loaded onto the gel. The method has a detection limit of 84.1 ng. We showed that the amount of GO in three different cell models, mouse macrophage cells (Raw264.7), human epithelial cells (A549) and mouse mesenchymal stem cells (MSC), could be accurately quantified by this assay, with the uptake rates decreasing in the order of MSC > Raw264.7 > A549. The results were consistent with the fluorescent imaging on cells exposed to fluorescence-labeled GO and TEM examination on ultrathin cell sections. The surprisingly highest uptake rate of MSC might be due to their abundant intracellular vesicles, which deserves further investigation. The novel method provides a complementary quantitative tool to the use of radioactive markers and fluorescent labeling of carbon nanomaterials and may facilitate the toxicological studies on carbon nanomaterials.