sp. nov., a bacterium isolated from marine sediment in the East China Sea and emended description of the genus .

A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated ZQ420, was isolated from marine sediment sampled on Zhoushan Island located in the East China Sea. Strain ZQ420 was able to grow at 10-45 °C, 0-12.0 % (w/v) NaCl and pH 5.5-9.0. Catalase and oxidase activities, nitrate reduction, HS production, hydrolysis of starch, casein, Tween 20, 40 and 80 were positive. Indole, methyl red, Voges-Proskauer test, hydrolysis of gelatin and Tween 60 were negative. The major cellular fatty acids were C 7, C and 11-methyl Cω7. Ubiquinone-10 (Q-10) was the only detected respiratory quinone. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids, two unidentified aminolipid and two unidentified lipids. The DNA G+C content was 64.8 mol%. According to 16S rRNA gene sequence similarities, strain ZQ420 shared 97.9, 96.3 and 96.3 % similarities to the following species with validated names D1-19, PAMC27389 and 4-T-34, respectively. While sharing lower sequence similarities (<96.0 %) to other type species. Phylogenetic analyses showed that strain ZQ420 and D1-19 formed an independent cluster in the phylogenetic trees. The average nucleotide identity value between strain ZQ420 and D1-19 was 79.1 %. The DNA-DNA hybridization analysis revealed that strain ZQ420 shared 21.5 % DNA relatedness with D1-19. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain ZQ420 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is ZQ420 (=KCTC 62579=MCCC 1K03530).