AI Article Synopsis
- Melanoma is a dangerous skin cancer with unclear implications of long noncoding RNAs (lncRNAs) in its progression; this study investigates their role using RNA-Seq data.
- The researchers discovered distinct gene expression patterns of lncRNAs in different stages of melanoma and found ZEB1-AS1 to be upregulated in melanoma cell lines, particularly in metastatic cases.
- ZEB1-AS1 expression correlates with higher invasiveness and positive links to ZEB1 gene expression, suggesting it may drive melanoma’s aggressive traits through an epithelial-to-mesenchymal transition (EMT)-like mechanism.
Article Abstract
Melanoma is the deadliest form of skin cancer, and little is known about the impact of deregulated expression of long noncoding RNAs (lncRNAs) in the progression of this cancer. In this study, we explored RNA-Seq data to search for lncRNAs associated with melanoma progression. We found distinct lncRNA gene expression patterns across melanocytes, primary and metastatic melanoma cells. Also, we observed upregulation of the lncRNA ZEB1-AS1 (ZEB1 antisense RNA 1) in melanoma cell lines. Data analysis from The Cancer Genome Atlas (TCGA) confirmed higher ZEB1-AS1 expression in metastatic melanoma and its association with hotspot mutations in BRAF (B-Raf proto-oncogene, serine/threonine kinase) gene and RAS family genes. In addition, a positive correlation between ZEB1-AS1 and ZEB1 (zinc finger E-box binding homeobox 1) gene expression was verified in primary and metastatic melanomas. Using gene expression signatures indicative of invasive or proliferative phenotypes, we found an association between ZEB1-AS1 upregulation and a transcriptional profile for invasiveness. Enrichment analysis of correlated genes demonstrated cancer genes and pathways associated with ZEB1-AS1. We suggest that the lncRNA ZEB1-AS1 could function by activating ZEB1 gene expression, thereby influencing invasiveness and phenotype switching in melanoma, an epithelial-to-mesenchymal transition (EMT)-like process, which the ZEB1 gene has an essential role.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683136 | PMC |
| http://dx.doi.org/10.1038/s41598-019-47363-6 | DOI Listing |
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