Background: Brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. gene is a good candidate for brucellosis vaccine and investigations showed that ( with several positive characteristic are attractive for protein expression as a live delivery vectors. These fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive.
Methods: gene was cloned in food grade pNZ 8149 vector and expressed in NZ 3900.
Results: Results showed that we can produce a food-grade recombinant producing the BP26 protein.
Conclusion: In this study, for Future evaluation about ability of as a live delivery vector, a food-grade recombinant producing the BP26 protein was produced.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626509 | PMC |
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