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| http://dx.doi.org/10.1111/pbi.13229 | DOI Listing |
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Establishment and application of Agrobacterium-delivered CRISPR/Cas9 system for wild tobacco () genome editing.
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Yunnan Academy of Tobacco Agricultural Sciences, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming, China.
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR-Cas9) system has been widely applied in cultivated crops, but limited in their wild relatives. is a typical wild species of genus that is globally distributed as a horticultural plant and well-studied as a self-incompatibility model. It also has valuable genes for disease resistance and ornamental traits.
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National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.
Chickpea is considered recalcitrant to in vitro tissue culture amongst all edible legumes. The clustered, regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9)-based genome editing in chickpea can remove the bottleneck of limited genetic variation in this cash crop, which is rich in nutrients and protein. However, generating stable mutant lines using CRISPR/Cas9 requires efficient and highly reproducible transformation protocols.
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Department of Horticulture, Chungnam National University, Daejeon 34134, Korea.
We determined the specificity of mutations induced by the CRISPR-Cas9 gene-editing system in tobacco () alleles and subsequent genetic stability. For this, we prepared 248 mutant plants using an -delivered CRISPR-Cas9 system targeting () and () genes, for which the mutation rates were 22.5% and 25%, respectively, with 20.
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Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat () cv. Fielder. Using -delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated.
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