Objective: Extensive drug resistant Gram-negative bacilli, harboring New Delhi metallo-β-lactamase-1 ( ) having the ability to hydrolyze β-lactams, have become a vital global clinical threat. The present study was, therefore, designed to investigate the prevalence and epidemiology of NDM-1 producers in Quetta, Pakistan.
Methods: This study was carried out in Microbiology Laboratory, Bolan Medical Complex Hospital Quetta, Biotechnology laboratory, BUITEMS Quetta and Hi-tech laboratory, CASVAB, University of Balochistan, Quetta, from March to June 2018, during the hot season. Biochemical and molecular approaches were applied for the identification of bacterial isolates. Minimum Inhibitory Concentrations (MICs) were determined using E-test method. Carbapenemase activity was ascertained by Modified Hodge Test (MHT) and the presence of gene was recognized by Polymerase Chain Reaction (PCR).
Results: We isolated five harboring isolates of three different species namely (n=2) (n=2) and (n=1), from 300 pus samples. These isolates were found extensive drug resistant (XDR). Strikingly, two isolates of . were displaying resistance against 23 antibiotics of sulphonamides, aminoglycosides, polypeptide, monobactams, tetracyclines, quinolones, macrolides, cephalosporins, phosphonic acid and β-lactams groups, suggesting Pan Drug Resistance (PDR).
Conclusion: This is the first report on emergence of PDR strain of producing NDM-1 in the province of Balochistan, Pakistan. The presence of in different bacterial species and their extensive rather pan drug resistance pattern poses a momentous clinical threat.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659055 | PMC |
http://dx.doi.org/10.12669/pjms.35.4.372 | DOI Listing |
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