AI Article Synopsis
- * Results showed that the treatment led to increased proliferation, migration, and altered morphology of human umbilical vein endothelial cells (HUVECs) when cultured on M13 phage-RGD coated surfaces.
- * Key factors like VEGF-A, VEGF receptors, MMP9, and eNOS were significantly upregulated, along with increased production of nitric oxide and reactive oxygen species (ROS), indicating enhanced vascularization potential.
Article Abstract
One of the most important goals of regenerative medicines is to generate alternative tissues with a developed vascular network. Endothelial cells are the most important cell type required in angiogenesis process, contributing to the blood vessels formation. The stimulation of endothelial cells to initiate angiogenesis requires appropriate extrinsic signals. The aim of this study was to evaluate the effects of M13 phage along with RGD peptide motif on in vitro and in vivo vascularization. The obtained results demonstrated the increased cellular proliferation, HUVECs migration, cells altered morphology, and cells attachment to M13 phage-RGD coated surface. In addition, the expression of Vascular Endothelial Growth Factor A (VEGF-A), VEGF Receptors 2 and 3, Matrix Metalloproteinase 9 (MMP9), and epithelial nitric oxide synthase (eNOS) transcripts were significantly upregulated due to the HUVECs culturing on M13 phage-RGD coated surface. Furthermore, VEGF protein secretion, nitric oxide, and reactive oxygen species (ROS) production were significantly increased in cells cultured on M13 phage-RGD coated surface.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6672002 | PMC |
| http://dx.doi.org/10.1038/s41598-019-47413-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Intra-abdominal transplantation of PLGA/PCL/M13 phage electrospun scaffold induces self-assembly of lymphoid tissue-like structure.
Biomed Pharmacother
April 2024
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:
Lymphoid organs are the main structural components of the immune system. In the current research, the mixture of poly lactic-co-glycolic acid (PLGA), polycaprolactone (PCL), and M13 phage or its RGD-modified form was used in the construction of a fibrillar scaffold using the electrospinning method. The constructs were transplanted intra-abdominally and examined for the formation of lymphoid-like tissues at different time intervals.
View Article and Find Full Text PDFM13 phage coated surface elicits an anti-inflammatory response in BALB/c and C57BL/6 peritoneal macrophages.
Int Immunopharmacol
June 2022
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:
Bacteriophages are one of the viral components of the human microbiome. M13 phages have recently been considered for immunotherapy because they can be detected by immune cells and stimulated immune responses. Macrophages are essential innate immune cells that respond to stimuli and direct subsequent immune responses.
View Article and Find Full Text PDFPromotion of angiogenesis by M13 phage and RGD peptide in vitro and in vivo.
Sci Rep
August 2019
Department of genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Article Synopsis
- * Results showed that the treatment led to increased proliferation, migration, and altered morphology of human umbilical vein endothelial cells (HUVECs) when cultured on M13 phage-RGD coated surfaces.
- * Key factors like VEGF-A, VEGF receptors, MMP9, and eNOS were significantly upregulated, along with increased production of nitric oxide and reactive oxygen species (ROS), indicating enhanced vascularization potential.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!