Blocking of Immunoblots and Incubation with Antibodies: Procedure for Immunoblots Prepared with Whole-Cell Lysates or Purified Proteins.

In this protocol, immunoblots are prepared for detection of the proteins of interest by incubation with blocking solution to prevent nonspecific antibody binding, followed by incubation with primary and secondary antibodies. When labeled primary antibody is used, incubation with secondary antibodies can be omitted.