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| http://dx.doi.org/10.1038/s41422-019-0209-9 | DOI Listing |
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OSP-1 protects neurons from autophagic cell death induced by acute oxidative stress.
Nat Commun
January 2025
Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, Australia.
Oxidative stress, caused by the accumulation of reactive oxygen species (ROS), is a pathological factor in several incurable neurodegenerative conditions as well as in stroke. However, our knowledge of the genetic elements that can be manipulated to protect neurons from oxidative stress-induced cell death is still very limited. Here, using Caenorhabditis elegans as a model system, combined with the optogenetic tool KillerRed to spatially and temporally control ROS generation, we identify a previously uncharacterized gene, oxidative stress protective 1 (osp-1), that protects C.
View Article and Find Full Text PDF[Research progress in optogenetic therapy for retinitis pigmentosa].
Zhonghua Yan Ke Za Zhi
January 2025
Shenzhen Eye Hospital, Jinan University, Shenzhen Institute of Eye Disease Control, Shenzhen518040, China.
Retinitis pigmentosa (RP) is a group of inherited retinal diseases characterized by progressive loss of photoreceptor cells and retinal pigment epithelium function. Its treatment has long been a focus and challenge in ophthalmic research. Despite advances in therapies such as stem cell transplantation, gene therapy, and retinal prosthetic implants, many difficulties remain.
View Article and Find Full Text PDFRecent advances in spatiotemporal control of the CRISPR/Cas9 system.
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December 2024
School of Life Sciences, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, Hubei 430042, China. Electronic address:
The CRISPR/Cas9 gene-editing technology, derived from the adaptive immune mechanisms of bacteria, has demonstrated remarkable advantages in fields such as gene function research and the treatment of genetic diseases due to its simplicity in design, precise targeting, and ease of use. Despite challenges such as off-target effects and cytotoxicity, effective spatiotemporal control strategies have been achieved for the CRISPR/Cas9 system through precise regulation of Cas9 protein activity as well as engineering of guide RNAs (gRNAs). This review provides a comprehensive analysis of the core components and functional mechanisms underlying the CRISPR/Cas9 system, highlights recent advancements in spatiotemporal control strategies, and discusses future directions for development.
View Article and Find Full Text PDFBlue Light-Induced, Dosed Protein Expression of Active BDNF in Human Cells Using the Optogenetic CRY2/CIB System.
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December 2024
Institute of Technical Chemistry, Leibniz University Hannover, Hannover, Germany.
The use of optogenetic tools offers an excellent method for spatially and temporally regulated gene and protein expression in cell therapeutic approaches. This could be useful as a concomitant therapeutic measure, especially in small body compartments such as the inner ear, for example, during cochlea implantation, to enhance neuronal cell survival and function. Here, we used the blue light activatable CRY2/CIB system to induce transcription of brain-derived neurotrophic factor (BDNF) in human cells.
View Article and Find Full Text PDFAnalysis of Light-Controlled Artificial Cell-Cell Adhesions.
Methods Mol Biol
December 2024
University of Münster Institute of Physiological Chemistry and Pathobiochemistry, Münster, Germany.
The precise spatial and temporal regulation of cell-cell adhesions is crucial for understanding the underlying biological processes and for assembling multicellular structures in tissue engineering. Traditional approaches have relied on chemical membrane functionalization and regulated gene expression of native cell adhesion molecules (CAMs), but these methods lack the necessary control and can be detrimental to cells. In contrast, engineered photoswitchable cell-cell adhesions offer a reversible and dynamic regulation at a single-cell resolution.
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