AI Article Synopsis
- * Researchers confirmed the presence of frizzled-4 in the human colon and obtained pure cultures of enteric neural progenitor cells using fluorescence-activated cell sorting (FACS) with frizzled-4 antibodies.
- * The isolated frizzled-4-positive cells were able to form neurosphere-like bodies and differentiate into functional neurons, demonstrating their potential in neuronal development through assays and patch-clamp analysis.
Article Abstract
Identification and isolation of neural progenitor cells from the human enteric nervous system (ENS) is currently hampered by the lack of reliable, specific markers. Here, we define the Wnt-receptor frizzled-4 as a marker for the isolation of enteric neural progenitor cells derived from paediatric gut samples. We show that the Wnt-receptor frizzled-4 is expressed in the human colon and in -derived enterospheres. To obtain a purified culture, we carried out fluorescence-activated cell sorting (FACS) using PE-conjugated frizzled-4 antibodies. Frizzled-4 cells gave rise to neurosphere-like bodies and ultimately differentiated into neurons as revealed by BrdU-proliferation assays and immunocytochemistry, whereas in frizzled-4 cultures we did not detect any neuronal and glial cells. By using a patch-clamp approach, we also demonstrated the expression of functional sodium and potassium channels in frizzled-4 cell cultures after differentiation in vitro.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721585 | PMC |
| http://dx.doi.org/10.3390/cells8080792 | DOI Listing |
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