Background: Reactive oxygen species (ROS) are involved in various cellular diseases. Excessive ROS can cause intracellular oxidative stress, resulting in a calcium imbalance and even aging. In this study, we evaluated the protective effect of esculetin on oxidative stress-induced aging in human HaCaT keratinocytes.
Methods: Human keratinocytes were pretreated with esculetin for 30 minutes and treated with HO. Then, the protective effects on oxidative stress-induced matrix metalloproteinase (MMP)-1 were detected by Flou-4-AM staining, reverse transcription-PCR, Western blotting, and quantitative fluorescence assay.
Results: Esculetin prevented HO-induced aging by inhibiting MMP-1 mRNA, protein, and activity levels. In addition, esculetin decreased abnormal levels of phospho-MEK1, phospho-ERK1/2, phospho-SEK1, phospho-JNK1/2, c-Fos, and phospho-c-Jun and inhibited activator protein 1 binding activity.
Conclusions: Esculetin prevented excessive levels of intracellular calcium and reduced the expression levels of aging-related proteins.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619853 | PMC |
| http://dx.doi.org/10.15430/JCP.2019.24.2.123 | DOI Listing |
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