Association of CYP1A1 M2 (A2455G) Polymorphism with Susceptibility to Breast Cancer in Mazandaran Province, Northern Iran: A Case-control Study.

Background: Breast cancer is one of the most frequent women malignancies in the world. The cytochrome P450 1A1 () is a key enzyme in xenobiotics metabolism. Moreover, CYP1A1 plays a critical role in the etiology of breast cancer by involving in 2-hydroxylation of estrogen. Therefore, single-nucleotide polymorphisms (SNPs) of its coding gene have been verified to be important in cancer susceptibility. The aim of the study was to evaluate the association of M2 (A2455G) includes rs1048943 of this SNP polymorphism with the risk of breast cancer in Mazandaran province.

Methods: Ninety-six breast cancer patients with known clinicopathological characters and 110 healthy women as control were genotyped for M2 polymorphisms by the restriction fragment length polymorphism technique.

Results: The analysis of gene (polymorphism M2) showed that the frequency of homozygous wild genotypes (AA), heterozygous (AG), and mutant genotype (GG) in the patient group, respectively, 78%, 22%, and 0%, and also the frequency of genotypes AA, AG, and GG in healthy included 82%, 16%, and 2%, respectively. Statistical analysis by Logistic regression model at < 0.05 showed no significant correlation between polymorphisms in 1A1M2 and breast cancer risk (odds ratio = 0.84, confidence interval = 0.33-2.17).

Conclusions: The results indicated that the M2 allelic genotypes were significantly associated neither with breast cancer risk nor with clinicopathological characteristics in Mazandaran province.