AI Article Synopsis
- Retinoids and rexinoids activate their nuclear receptors, influencing the expression of target genes, making mRNA levels a key measure of their activity.
- These changes in mRNA expression are important for biomarker trials and preclinical studies.
- Assessing the quality of RNA extracted from archival tissues is crucial, especially with the rise of next generation sequencing for gene expression profiling.
Article Abstract
Retinoids and rexinoids directly and selectively activate their nuclear receptors, resulting in changes in the transcript levels of their target genes. Consequently, quantitating mRNA levels transcribed from cognate target genes is the most accurate measure of retinoid action. These changes can serve as relevant endpoints in biomarker trials, as well as in vivo preclinical studies. In gene expression analyses of archival material such as formalin-fixed paraffin-embedded (FFPE) tissues, assessing the quality of the extracted RNA is essential for the validation of the studies. With next generation sequencing (NGS) becoming the method of choice for gene expression profiling, RNA quality has become a critical aspect of study feasibility. In this chapter, we describe a method to extract RNA and to assess the intactness of RNA samples extracted from paraffin-embedded tissues.
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| http://dx.doi.org/10.1007/978-1-4939-9585-1_16 | DOI Listing |
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