A simple time-resolved fluorescence assay for quantitative determination of DOTA chelator.

DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate) is one of the preeminent metal chelator applied for diagnostic and therapeutic purposes, however to date there is no versatile and reliable nonradioisotopic method for its precise determination. In this technical note, we present a novel and sensitive fluorimetric assay for quantitative determination of DOTA based on the luminescence quenching of the highly luminescent europium ions complex with trioctyl phosphine oxide and naphthoyl trifluoroacetone sensitizing activators. The assay is carried out in two simple steps and enables the determination of DOTA in the nanomolar range providing a superior tool compared to commonly applied spectrophotometric assay with Arsenazo-III reagent.