Mutations in Drosophila melanogaster thi2 strain obtained using exogenous viral DNA, and in the thi2 derivatives, have been studied. Revertants to the wild type arose with high frequency (1-2.10(-2)) in both 83f-7 (1-2.6-5.3) and the initial thi2 (2-71.4) strains. New mutations of chromosome 2, non-allelic to the thi2, though with thi2-like phenotypic effects, and some lethal alleles of these mutations have been found in the progeny of the revertants from the thi2 to the wild type in 83f-7 strain. Cytological analysis has failed to disclose large chromosome aberrations of salivary gland chromosomes carrying the mutations r83f-3 (1-54.5), 83f-7, 83f-21 (3-67.1) originated from the thi2 strain. The data are consistent with possibility that mutations from the thi2 strain and its derivatives were formed by means of a small insertion sequence.
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