AI Article Synopsis
- Proteins are flexible and dynamic rather than rigid, making it important to study their behavior in physiological conditions.* -
- The technique of hydrogen deuterium exchange coupled with mass spectrometry (HDX-MS) is introduced as a valuable method to explore protein dynamics, with case studies involving nuclear and innate immunity receptors.* -
- Recent advancements in software are improving data analysis and visualization of HDX-MS results, enhancing our understanding of protein structure and function.*
Article Abstract
Proteins are not rigid bodies under their physiological conditions. Here we discuss a solution-phase structural proteomics technique, hydrogen deuterium exchange coupled with mass spectrometry (HDX-MS), as a means to study protein dynamics, which can complement other structural approaches. We outline the background theory and highlight the utility of HDX-MS measurements in two case studies involving a nuclear receptor and an innate immunity receptor. We also discuss emerging software advances for improving data analysis and three-dimensional visualization.
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| http://dx.doi.org/10.1016/j.sbi.2019.06.007 | DOI Listing |
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