Background: is an emergent Rickettsial agent whose main vector is , but ticks, mites and lice are also infected. We aimed to search for molecular evidence of spp. in fleas collected from dogs and wild rodents (Heteromys anomalous) from three villages of Córdoba and Antioquia provinces (Northern of Colombia), where outbreaks of rickettsioses have occurred, and discuss the possible role of fleas on endemic/enzootic regions for rickettsia.
Methods: During 2010 and 2012, 649 and 24 fleas were removed from dogs and wild rodents (), respectively, in 3 locations from Córdoba and Antioquia provinces (Colombia). These fleas were tested into pools for Rickettsial infection by PCR, targeting , and Rickettsial genes.
Results: Almost 20% (30/153) of pools contained Rickettsial DNA. The fragments of gene showed high identity values between sequences from Necocli and Los Cordobas with strain from Senegal (100% and 99.7% respectively) and all were highly related by phylogenetic analyses. Rickettsial DNA in pools of was not detected.
Conclusion: Our findings highlighted the endemicity of the infection by in fleas from northern of Colombia and showed the likely importance of dogs as hosts of fleas and their potential role as reservoirs of
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