Premise: The natural population size of (Lycopodiaceae) has dramatically decreased and the species has become endangered due to overexploitation. Here, we developed simple sequence repeat (SSR) markers for to survey both its genetic diversity and population structure.
Methods And Results: Based on 177 individuals, 120 SSR primer pairs were developed and optimized from five regions of the transcriptomic data. Of these primer pairs, 20 were successfully amplified and 10 showed obvious polymorphism. These polymorphic loci were investigated to study the genetic diversity of . Two to 11 alleles per locus were identified, the level of observed heterozygosity ranged from 0.00 to 1.00, and the level of expected heterozygosity ranged from 0.19 to 0.79. All loci were successfully amplified in , , and .
Conclusions: The 10 polymorphic primer pairs developed here will be valuable for studies of the endangered and other related species.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636615 | PMC |
| http://dx.doi.org/10.1002/aps3.11273 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Rapid detection of by recombinase-aided amplification combined with the CRISPR/Cas12a system.
Front Cell Infect Microbiol
December 2024
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
() is one of the primary agents involved in porcine respiratory disease complex, and circulates in the swine industry worldwide. The prevention and control of is complicated. Thus, a recombinase-aided amplification (RAA) assay coupled with the clustered regularly-interspaced short palindromic repeats (CRISPR)/Cas12a system was established for the detection of .
View Article and Find Full Text PDFDevelopment of a SYBR Green Real-Time PCR method for the detection of and using chloroplast genes.
Food Sci Biotechnol
January 2025
Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon, Republic of Korea.
Unlabelled: (Arabica) and (Robusta) are valuable agricultural products traded worldwide. In this study, we designed specific primer pairs for Arabica and Robusta using chloroplast genes to distinguish and quantify the two types of coffee beans. We assessed the specificity, sensitivity, and applicability of the qRT-PCR assay using all the primer pairs.
View Article and Find Full Text PDFDevelopment of dual polymerase spiral reaction for detection of Listeria monocytogenes and Staphylococcus aureus simultaneously.
Int J Food Microbiol
January 2025
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, China. Electronic address:
Listeria monocytogenes and Staphylococcus aureus are prevalent foodborne pathogens responsible for poisoning humans with food. The present study was devoted to the establishment of a method based on dual polymerase spiral reaction (dual-PSR) and melting curve analysis for concurrent identification L. monocytogenes and S.
View Article and Find Full Text PDFGenome-wide development of simple sequence repeat (SSR) markers at 2-Mb intervals in lotus (Nelumbo Adans.).
BMC Genomics
January 2025
Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, No. 3888 Chenhua Road, Songjiang District, Shanghai, 201602, China.
Background: Despite the rapid advancement of high-throughput sequencing, simple sequence repeats (SSRs) remain indispensable molecular markers for various applied and research tasks owing to their cost-effectiveness and ease of use. However, existing SSR markers cannot meet the growing demand for research on lotus (Nelumbo Adans.) given their scarcity and weak connections to the lotus genome.
View Article and Find Full Text PDFFirst Report of Causing Root Rot of Incense Cedar in Tennessee and the United States.
Plant Dis
January 2025
Tennessee State University, Otis Floyd Nursery Research Center, 472 Cadillac Lane, McMinnville, Tennessee, United States, 37110;
Incense cedar [ (Torr.) Florin] is a coniferous evergreen tree, indigenous to western North America, that is being evaluated in Tennessee for its adaptability to eastern U.S.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!