Molecular analysis and antimicrobial resistance pattern of distinct strains of isolated from cystic fibrosis patients in Iran.

Background And Objectives: Colonization of in Cystic Fibrosis (CF) patients may lead to severe pulmonary disease and death. Different characteristics of from these patients were determined in the present study.

Materials And Methods: Antimicrobial susceptibility and AmpC-overproduction were determined. The β-lactamase genes were detected by PCR and the gene was sequenced in some of the carbapenem resistance isolates. Distribution of genes was determined by PCR. Cytotoxicity of Exo effector proteins was measured using A549 cells. Biofilm production was determined by microtiter plate assay. Random amplified polymorphic DNA (RAPD) -PCR was performed for molecular analysis.

Results: Polymyxin B, piperacillin/tazobactam and meropenem were the most active antibiotics and 9.6% of isolates were overproducers. The prevalence of , , , and genes were as follow: 22.7%, 3.75%, 6.25% and 3.75%, respectively. A high proportion (83.5%) of isolates was able to produce biofilm. The gene was present in all isolates while was present in about 35% of them. RAPD-PCR revealed 49 patterns among 78 tested isolates in which 34 patterns were detected once.

Conclusion: Biofilm formation ability and relatively high frequency of may contribute to the persistence of bacteria within lungs of CF patients. Some characteristics of isolates recovered from a single patient after several sampling procedures were similar, while others lacked resemblance.