Molecular docking, QSAR properties and DNA/BSA binding, anti-proliferative studies of 6-methoxy benzothiozole imine base and its metal complexes.

The molecular and QSAR (Quantitative Structure-Activity Relationship) properties of title compound 2-((6-Methoxybenzo[d]thiazol-2-ylimino)methyl)-6-ethoxyphenol (HL) were evaluated employing HyperChem 7.5 tools. The interaction of the complexes of HL with calf thymus DNA (CT-DNA) was investigated by absorption titrations, Fluorescence quenching and viscosity measurements. The experimental data suggest that these complexes bind to CT-DNA through an intercalative mode, wherein DNA-binding affinity of is found to be greater compared to other complexes. The tryptophan emission-quenching with bovine serum albumin (BSA) experiment revealed stronger binding of than other complexes in the hydrophobic region of protein. The photocleavage of plasmid pBR322 DNA investigated in the presence of the title complexes inferred conversion of supercoiled form of DNA plasmid to circular nicked form. Free-radical scavenging activity studies of HL and its metal complexes determined by their interaction with the stable free-radical DPPH have shown promising antioxidant property. Further cytotoxicity studies with HeLa and MCF-7 cell lines indicated that the compounds can efficiently inhibit the cell proliferation in a dose dependent manner. The DAPI staining assay studies revealed the higher potency of to induce apoptosis. AbbreviationsBSABovine serum albumin proteinCT-DNACalf thymus DNADMSODimethyl sulfoxideDAPI4',-6-Diamidino-2-phenylindole dihydrochlorideESI-MSElectrospray ionization mass spectrometryICHalf-maximal inhibitory concentrationMBTYE2-((6-methoxybenzo[d]thiazol-2-ylimino) methyl)-6-ethoxyphenolMTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidePBSPhosphate-buffered salineTrisTris(hydroxymethyl)aminomethaneCommunicated by Ramaswamy H. Sarma.