AI Article Synopsis
- CD4 T lymphocytes can differentiate into various subtypes like Th1, Th2, Th17, and Treg cells based on immune scenarios.
- The study explored how synthetic conotoxins from marine snails impacted the proliferation and differentiation of these T cell subpopulations in cultured BALB/c mice lymphocytes.
- Findings revealed that conotoxins cal14.1b and cal14.2c increased IL-10 production in Treg cells while decreasing IL-17 levels but did not affect T cell proliferation or the Th1/Th2 balance, suggesting their immunomodulatory potential.
Article Abstract
CD4 T lymphocytes are able to differentiate into distinct subtypes according to several immunological scenarios, including T helper (Th)1, Th2, Th17 and regulatory T (Treg) cells. CD4 T cells are phenotypically flexible and have specific ion channels, such as the nicotinic acetylcholine receptors (nAChR) that could be modulated by peptides produced by marine snails, known as conotoxins. Their effect on T lymphocytes has not been explored and emerging evidence suggests that these peptides may have immunomodulatory activities. This study investigated the effect of two -derived synthetic conotoxins on the proliferation and differentiation of T lymphocyte subpopulations Th1, Th2, Th17 and Treg. Cells from lymph nodes of BALB/c mice were cultured in the presence of conotoxins cal14.1b and cal14.2c (5.5 μM), during 96 h. Cell proliferation and intracellular cytokine production (IFN-γ, IL-4, IL-17 and IL-10) were analyzed by flow cytometry. cal14.1b and cal14.2c increased intracellular IL-10 production in Treg (CD3CD4Foxp3) cells and decreased intracellular IL-17 production (CD3CD4) after 72 h of culture. Conotoxins did not show any effect on T cell proliferation nor Th1/Th2 balance. These results suggest that synthetic conotoxins exert immunomodulatory activity, especially by regulating specific functions on T lymphocytes.
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| http://dx.doi.org/10.1080/08923973.2019.1641114 | DOI Listing |
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