A repetitive sequence-based polymerase chain reaction (rep-PCR) technique utilizing a semiautomated system, namely DiversiLab, was applied to determine the genotypes of and obtained from slaughterhouses. Twenty-four and 16 isolates from pigs and Hanwoo cattle from three slaughterhouses were used to create a DNA fingerprint library with the system software. Scatterplots demonstrated that rep-PCR groupings of isolates were in good agreement with their origins. Specifically, linked rep-PCR profiles were observed for isolates recovered from the same slaughterhouse, and higher genetic similarities were found among strains isolated from adjacent regions. All isolates except one (ID: A-Hanwoo-9) from slaughterhouse "A" clustered with the three reference strains, Korea Culture Center of Microorganisms (KCCM) 41291, KCCM 12214, and Culture Collection of Antimicrobial Resistant Microbes (CCARM) 3A007 (similarity values >95%). Moreover, most isolates obtained from slaughterhouse "B" clustered with KCCM 11335 and KCCM 41331, and two isolates from slaughterhouse "C" clustered with CCARM 0027. Therefore, for this species, genotypic characteristics of regional isolates can be used to track the pathway of contamination. In contrast, isolates showed high genetic diversity and could not be clustered with any specific group. Collectively, this system is useful for analyzing genetic diversity and is a rapid and reproducible typing method; however, there is a need to develop rep-PCR libraries for its use as a rapid identification method.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/fpd.2019.2619 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genomic insights into drug resistance and virulence determinants in rare pyomelanin-producing clinical isolates of Acinetobacter baumannii.
Eur J Clin Microbiol Infect Dis
December 2024
Department of Biotechnology, School of Life Sciences, Pondicherry University, R. Venkataraman Nagar, Kalapet, Pondicherry, 605014, India.
Purpose: Clinical isolates of multi-drug resistant Acinetobacter baumannii are a major cause of nosocomial infections, often attributed to the highly adaptable genome that helps it to thrive under environmental selection pressure. Here, we aim to provide genotypic-based surveys and comparative whole genome sequencing (WGS) analysis to explore the genomics of the rare pyomelanin-forming clinical isolates of A. baumannii from India.
View Article and Find Full Text PDFApplicability of AlphaFold2 in the modeling of dimeric, trimeric, and tetrameric coiled-coil domains.
Protein Sci
January 2025
Institute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Warsaw, Poland.
Coiled coils are a common protein structural motif involved in cellular functions ranging from mediating protein-protein interactions to facilitating processes such as signal transduction or regulation of gene expression. They are formed by two or more alpha helices that wind around a central axis to form a buried hydrophobic core. Various forms of coiled-coil bundles have been reported, each characterized by the number, orientation, and degree of winding of the constituent helices.
View Article and Find Full Text PDFBiofilm formation, antibiotic-resistance and clonal relatedness among clinical isolates of Acinetobacterbaumannii.
Microb Pathog
October 2024
Laboratory of Alavi Educational Hospital, Ardabil University of Medical Sciences, Ardabil, Iran.
In this work, the antibiotic resistance, biofilm formation capability, and clonal relatedness of 50 A. baumannii isolates collected from three hospitals in Ardabil city, Iran, were evaluated. Antibiotic sensitivity and biofilm formation of isolates were determined by disk diffusion and microtiter-plate methods, respectively.
View Article and Find Full Text PDFPhylogenetic group, antibiotic resistance, virulence gene, and genetic diversity of causing bloodstream infections in Iran.
Front Microbiol
July 2024
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
is one of the most important pathogens causing bloodstream infections (BSIs) throughout the world. We sought to characterize the phylogroup classification, major human sequence types (STs), antimicrobial resistance, presence of selected antimicrobial resistance and virulence genes, and genetic diversity of isolated from patients with BSIs at the University Hospital in Iran. A total of 100 bloodstream isolates were collected between December 2020 and June 2022.
View Article and Find Full Text PDFMobile genetic element-driven genomic changes in a community-associated methicillin-resistant clone during its transmission in a regional community outbreak in Japan.
Microb Genom
July 2024
Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
Article Synopsis
- - Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are rising as a public health concern, particularly in community settings like maternity clinics, with a specific outbreak in Japan identified through analysis techniques.
- - A study analyzed 151 CA-MRSA isolates, identifying 133 related to the outbreak, which belonged to a lineage called the TDC clone, part of the clonal complex 30 (SWP clone).
- - The research highlighted that the TDC clone had been present before the outbreak, showing long persistence in carriers, high transmission within households, and significant genetic changes tied to mobile genetic elements that affected antibiotic resistance.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!