Efficacy of nanoencapsulated pelargonidin in ameliorating pesticide toxicity in fish and L6 cells: Modulation of oxidative stress and signalling cascade.

Removal of bio-accumulated pesticides in edible fish is a global problem. In this study, we tested protective capability of a phytochemical pelargonidin-loaded non-toxic, biodegradable poly-lactide-co-glycolide nano-particles (NPG) against toxicity induced by a pesticide cypermethrin (CM) in a fish model (Oreochromis mossambica) in vivo and also in L6 muscle cell line, in vitro. First we assessed potential sustainable release of nanoparticles following oral administration of NPG to fish, their ability to cross sub-cellular membranes in several tissues and efficacy to cross blood-brain-barrier. Next, protective ability of NPG, if any, against CM in fish was evaluated deploying parameters like % cell viability, DNA damage in muscle cells and modulation of anti-oxidative-enzymes like superoxide dismutase, catalase and lipid peroxidase. Modulation of reactive oxygen species generation, nuclear condensation and alteration in stress related protein signalling cascade were assessed in L6 cells. Results revealed that NPG had nano-size range (~10-12 nm) and negative zeta potential (-17 mV). Bioavailability and distribution of NPG could be followed by spectrophotometric absorbance of pelargonidin at 293 nm from 6 h onward till 24 h in all important tissues including the brain. Thus, 0.5 mg/g b.w. NPG could demonstrate protective ability in CM-intoxicated fish muscle cells in respect of % cell viability, DNA damage and stress related enzymes. Similar alterations could also be found in signalling protein cascade in L6 cells in response to treatment of 5 μg/ml NPG against CM-induced toxicity and depletion of overall ROS generation and nuclear condensation. Therefore, NPG could be used as a potential drug in management of pesticide toxicity in cultured edible fish.