Wildly grown in most regions of the world, is a highly underutilised fruit with significant antioxidant characteristics. The phyto and physicochemical properties of berries at different stages of ripening are evaluated in this work. Total flavonoids (TF), total phenolic content (TPC) and antioxidant activity were determined spectrophotometrically, while concentration of polyphenols was determined using liquid chromatography coupled to diode array detection and electrospray ionization mass spectrometry. Results showed that antioxidant activity was lowest (18.36 ± 0.12 mg TE/g) in RS3 and decreased with TPC upon increased ripening. Conversely, TF increased with ripening progression with TF found to be highest in RS3 (5.92 ± 0.03 mg CE/g). Identified phenolic acids in were quinic acid, protocatechuoyl-hexose, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and dicaffeoylquinic acid. Identified flavonoids included rutin, catechin, procyanidin dimer, procyanidin trimer, quercetin-3--glucosyl-xyloside, quercetin-3--robinobioside, quercetin-3--glucoside and quercetin-3-OH-3-methylglutaryl-glucoside. Physicochemical properties of varied among samples with sugar/acid ratio of ranging from 25.70 for RS1 to 50.36 for RS3. Ripening stage of undoubtedly affects the phyto and physicochemical properties of .
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| http://dx.doi.org/10.3390/molecules24142630 | DOI Listing |
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