Identification of a functional nuclear localization signal in 3D/3CD of duck hepatitis A virus 1.

Virus Res

College of Veterinary Medicine, Shandong Agricultural University, Shandong Taian, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, 271018, China. Electronic address:

Published: September 2019

The nuclear localization signals (NLS) were usually composed of basic residues (K and R) and played an important role in delivery of genomes and structural protein into nucleus. In this research, we identified that 3D/3CD entered into nucleus during viral propagation of duck hepatitis A virus type 1 (DHAV-1). To investigate the reason that 3D/3CD entered into nucleus, the amino acid sequence of 3CD was analyzed through NLS Mapper program. The basic region PRKTAYMRS was subsequently proved to be a functional NLS to guide 3D/3CD into nucleus. R, K and R were found essential for maintaining the nuclear targeting activity, and exchange between R and K had no impact on cellular localization of 3D. Since the entry of 3D/3CD into nucleus was essential for shutoff of host cell transcription and maintaining the viral propagation of picornavirus numbers, our study provided new insights into the mechanism of DHAV-1 propagation.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.virusres.2019.197670DOI Listing

Publication Analysis

Top Keywords

nuclear localization
8
duck hepatitis
8
hepatitis virus
8
3d/3cd entered
8
entered nucleus
8
viral propagation
8
3d/3cd nucleus
8
nucleus essential
8
3d/3cd
5
nucleus
5

Similar Publications

Molecular imaging using positron emission tomography (PET) provides sensitive detection and mapping of molecular targets. While cancer-associated fibroblasts and integrins have been proposed as targets for imaging of pancreatic ductal adenocarcinoma (PDAC), herein, spatial transcriptomics and proteomics of human surgical samples are applied to select PDAC targets. We find that selected cancer cell surface markers are spatially correlated and provide specific cancer localization, whereas the spatial correlation between cancer markers and immune-related or fibroblast markers is low.

View Article and Find Full Text PDF

Proteostasis is maintained through regulated protein synthesis and degradation and chaperone-assisted protein folding. However, this is challenging in neuronal projections because of their polarized morphology and constant synaptic proteome remodeling. Using high-resolution fluorescence microscopy, we discover that hippocampal and spinal cord motor neurons of mouse and human origin localize a subset of chaperone mRNAs to their dendrites and use microtubule-based transport to increase this asymmetric localization following proteotoxic stress.

View Article and Find Full Text PDF

Mechanical Force-Induced cGAS Activation in Carcinoma Cells Facilitates Splenocytes into Liver to Drive Metastasis.

Adv Sci (Weinh)

December 2024

Department of General Surgery, National-Local Joint Engineering Research Center of Biodiagnostic & Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China.

Liver metastasis is the main cause of cancer-related mortality. During the metastasis process, circulating carcinoma cells hardly pass through narrow capillaries, leading to nuclear deformation. However, the effects of nuclear deformation and its underlying mechanisms on metastasis need further study.

View Article and Find Full Text PDF

TabHLH489 suppresses nitrate signaling by inhibiting the function of TaNLP7-3A in wheat.

J Integr Plant Biol

December 2024

The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education; Shandong Key Laboratory of Precision Molecular Crop Design and Breeding; School of Life Sciences, Shandong University, Qingdao, 266237, China.

Nitrate not only serves as the primary nitrogen source for terrestrial plants but also serves as a critical signal in regulating plant growth and development. Understanding how plant responses to nitrate availability is essential for improving nitrogen use efficiency in crops. Herein, we demonstrated that the basic helix-loop-helix (bHLH) transcription factor TabHLH489 plays a crucial negative regulatory role in wheat nitrate signaling.

View Article and Find Full Text PDF

Background: Coronary Artery Disease (CAD) is a leading cause of mortality, with an increasing number of patients affected by coronary artery stenosis each year. Coronary angiography (CAG) is commonly employed as the definitive diagnostic tool for identifying coronary artery stenosis. Physician Visual Assessment (PVA) is often used as the primary method to determine the need for further intervention, but its subjective nature poses challenges.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!