AI Article Synopsis
- The study investigates the link between sperm DNA damage and protamine deficiency in men with oligospermia, asthenospermia, and teratospermia, compared to normospermic controls.
- Significant differences in sperm protamine deficiency were found among the infertility groups, especially with negative correlations in oligospermia and asthenospermia regarding sperm motility.
- The results suggest that standard sperm analysis might need to include chromatin quality tests for better assessments of male infertility, as motor performance alone doesn’t guarantee sperm DNA integrity.
Article Abstract
Objective: Abnormality in Histone-Protamine replacements has been indicated to cause sperm DNA damage and infertility. The aim of the present study was to investigate the relationships between sperm parameters in oligospermia, asthenospermia, and teratospermia with protamine deficiency in infertile men.
Material And Method: In this case-control study, we had three experimental groups including oligospermia (n=100), asthenospermia (n=100), and teratospermia (n=100) as well as normospermia (n=100) as controls. Sperm analyses were performed according to the recommendations of the World Health Organization (WHO, 2010) and sperm chromatin quality was assessed using Chromomycin A3 (CMA3) staining for each sample.
Results: The comparison of the data between groups indicated that the percentage of spermatozoa with protamine deficiency was significantly different in patients with oligospermia, asthenospermia, and teratospermia when compared with control ones. However, there was no significant correlation between sperm nuclear protamine deficiency and their parameters of the men with teratospermia using CMA3 test. Regarding the oligospermia and asthenospermia semen samples, the findings showed the negative correlations between the sperm nuclear protamine deficiency and progressive motility as well as immobility (p<0.001).
Conclusion: The higher proportion of spermatozoa with abnormal chromatin packaging was observed in asthenospermic samples than those from other experimental groups as well as controls. It seems that normal morphology cannot have a valuable predictive value for good chromatin quality of spermatozoa, as much as normal motility characteristics, since samples with high mobility rates often have lower protamine deficiencies. The findings may provide a supportable promoting the future wider clinical application of chromatin/DNA integrity testing along with the semen analysis in male infertility.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.androl.2019.04.003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Correlation of sperm motility, acrosome integrity, protamine deficiency, and DNA fragmentation in proven and unproven Friesian Holstein bulls.
J Adv Vet Anim Res
September 2024
Research Center for Applied Zoology, National Research and Innovation Agency, Bogor, Indonesia.
Article Synopsis
- This study evaluates the frozen semen quality of Friesian Holstein bulls to assess male fertility for artificial insemination, comparing proven and unproven bulls based on various scientific analyses.
- Six proven and six unproven bulls' semen was tested using advanced techniques like Computer Assisted Semen Analysis and staining methods to measure parameters like sperm motility, viability, and DNA integrity.
- Results showed no significant differences in frozen semen quality between proven and unproven bulls, indicating that both types are equally viable for breeding programs and highlighting the importance of molecular analysis in assessing semen health.
EP300-interacting inhibitor of differentiation 3 is required for spermatogenesis in mice.
Andrology
November 2024
Department of Histology and Embryology, School of Basic Medical Sciences, State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, China.
Background: Mammalian spermatogenesis is a highly complex process of cell proliferation, meiosis, and differentiation. A series of genes are expressed in an orderly and precise manner to ensure spermatogenesis, with chromatin undergoing intricate changes throughout. EP300-interacting inhibitor of differentiation 3 (Eid3) is a testis-enriched gene, but its role in male reproduction remains unclear.
View Article and Find Full Text PDFProtamine 2 deficiency results in Septin 12 abnormalities.
Front Cell Dev Biol
October 2024
Laboratory of Reproductive Biology, Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Czechia.
There is a well-established link between abnormal sperm chromatin states and poor motility, however, how these two processes are interdependent is unknown. Here, we identified a possible mechanistic insight by showing that Protamine 2, a nuclear DNA packaging protein in sperm, directly interacts with cytoskeletal protein Septin 12, which is associated with sperm motility. Septin 12 has several isoforms, and we show, that in the sperm, the short one (Mw 36 kDa) is mis-localized, while two long isoforms (Mw 40 and 41 kDa) are unexpectedly lost in sperm chromatin-bound protein fractions.
View Article and Find Full Text PDFEffects of alpha-lipoic acid and sildenafil citrate on sperm quality in asthenozoospermic men during freezing-thawing processes.
Cryobiology
December 2024
Department of Biology, Faculty of Science, Arak University, Arak 3815688138, Iran. Electronic address:
Article Synopsis
- The study investigated how alpha-lipoic acid (ALA) and sildenafil citrate (SC) affect sperm quality during freeze-thawing in men with low sperm motility (asthenozoospermia).
- Thirty semen samples were categorized into five groups: fresh, freeze, freeze + SC, freeze + ALA, and freeze + SC + ALA, and various sperm parameters were analyzed.
- The results showed that the freeze process harmed sperm quality, but adding SC and ALA significantly improved sperm viability, motility, and other quality indicators while reducing oxidative stress and inflammation.
Effects of Acute Exposure to Methylglyoxal or/and A Diet Rich in Advanced Glycation End Products on Sperm Parameters in Mice.
Int J Fertil Steril
June 2024
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Email:
Background: Advanced glycation end products (AGEs) that accompany many metabolic disorders including diabetes, obesity, and a wide range of dyslipidemia conditions, are strongly associated with adverse effects on cell and tissue homeostasis. Accordingly, our objective was to investigate the impact of AGE-promoting diets on mouse models, considering both scenarios with and without methylglyoxal (MGO) as a primary precursor of AGEs.
Materials And Methods: In this experimental study, 5-week-old C57BL/6 mice were split into four groups as a control group (n=5), AGE (n=5), MGO (n=8), and AGE-MGO-diets (n=8).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!