Background: The allotetraploid tobacco species Nicotiana benthamiana native to Australia has become a popular host for recombinant protein production. Although its usage grows every year, little is known on this plant's genomic and transcriptomic features. Most N. benthamiana accessions currently used in research lack proper documentation of their breeding history and provenance. One of these, the glycoengineered N. benthamiana line ΔXT/FT is increasingly used for the production of biopharmaceutical proteins.
Results: Based on an existing draft assembly of the N. benthamiana genome we predict 50,516 protein -encoding genes (62,216 transcripts) supported by expression data derived from 2.35 billion mRNA-seq reads. Using single-copy core genes we show high completeness of the predicted gene set. We functionally annotate more than two thirds of the gene set through sequence homology to genes from other Nicotiana species. We demonstrate that the expression profiles from leaf tissue of ΔXT/FT and its wild type progenitor only show minimal differences. We identify the transgene insertion sites in ΔXT/FT and show that one of the transgenes was inserted inside another predicted gene that most likely lost its function upon insertion. Based on publicly available mRNA-seq data, we confirm that the N. benthamiana accessions used by different research institutions most likely derive from a single source.
Conclusions: This work provides gene annotation of the N. benthamiana genome, a genomic and transcriptomic characterization of a transgenic N. benthamiana line in comparison to its wild-type progenitor, and sheds light onto the relatedness of N. benthamiana accessions that are used in laboratories around the world.
Lily plants, known for their colorful flowers and strong scent, displayed yellow mottling and mosaic symptoms in March 2024 during an outbreak in Hidalgo County, Texas.
Despite initial negative tests for potyvirus using commercial diagnostics, further investigations revealed a mechanically transmissible agent was present, as symptoms emerged on test plants inoculated with samples from affected lilies.
DNA analysis confirmed the presence of lily mottle virus (LMoV), identified through PCR amplification and sequencing, showing a significant identity match with known sequences of potyvirus.
The para rubber tree (Hevea brasiliensis) is the world's sole commercial source of natural rubber, a vital industrial raw material. However, the narrow genetic diversity of this crop poses challenges for rubber breeding. Here, we generate high-quality de novo genome assemblies for three H.
Ningbo University, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, 818 Fenghua Road, Ningbo, China, 315211;
Potato virus H (PVH) was first discovered in potatoes in Inner Mongolia, China and has since been noted to infect other plants like pepino.
A recent study in Yunnan, China, observed typical viral symptoms in tomato plants during an open field survey, leading to the collection of 11 symptomatic samples for further analysis.
High-throughput sequencing identified the presence of three viruses in the tomato samples, including PVH, using advanced RNA sequencing techniques and bioinformatics to locate and characterize the pathogens.
Background: Septoria tritici blotch (STB), caused by the foliar fungus Zymoseptoria tritici, is one of the most damaging disease of wheat in Europe. Genetic resistance against this fungus relies on different types of resistance from non-host resistance (NHR) and host species specific resistance (HSSR) to host resistance mediated by quantitative trait loci (QTLs) or major resistance genes (Stb). Characterizing the diversity of theses resistances is of great importance for breeding wheat cultivars with efficient and durable resistance.
