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Biomimetic dentin remineralization using eggshell derived nanohydroxyapatite with and without carboxymethyl chitosan - An in vitro study.
Int J Biol Macromol
June 2024
Department of Conservative Dentistry and Endodontics, SRM Dental College, Bharathi Salai, SRM Institute of Science and Technology, Chennai 600 089, India. Electronic address:
The objective of this study was to evaluate the synergistic effect of eggshell-derived nanohydroxyapatite (EnHA) and carboxymethyl chitosan (CMC) in remineralizing artificially induced dentinal lesions. EnHA and CMC were synthesized using simple chemical processes and characterized using FTIR, XRD, HRSEM-EDX, TEM, DLS and TGA/DTA analyses. A total of 64 pre-demineralized coronal dentin specimens were randomly subjected to following treatments (n = 16):artificial saliva (AS), EnHA, CMC, and EnHA-CMC, followed by pH cycling for 7 days.
View Article and Find Full Text PDFCalcined chicken eggshell-derived biomimetic nano-hydroxyapatite as a local drug-delivery aid for doxycycline hyclate: characterization, bio-activity, cytotoxicity, antibacterial activity and release study.
RSC Adv
December 2023
Institute of Glass and Ceramic Research and Testing (IGCRT), Bangladesh Council of Scientific and Industrial Research (BCSIR) Dhaka-1205 Bangladesh
The integration of bioactive substances with antibiotics has been extensively pursued for the treatment of osteomyelitis. These materials, also known as biomaterials, can serve both as bone replacements and targeted drug delivery systems for antibiotics. In this study, biomimetic nano-hydroxyapatite (nHAp) was synthesized the coprecipitation technique where waste chicken eggshell (WCE) was employed as the source of Ca.
View Article and Find Full Text PDFMicro-Raman spectroscopy analysis of dentin remineralization using eggshell derived nanohydroxyapatite combined with phytosphingosine.
J Mech Behav Biomed Mater
May 2023
Department of Physics, Easwari Engineering College, Ramapuram, Chennai, 600 089, Tamil Nadu, India.
The aim of this study was to assess the remineralization efficacy of chicken eggshell-derived nano-hydroxyapatite (CEnHAp) combined with phytosphingosine (PHS) on artificially induced dentinal lesions. PHS was commercially procured whereas CEnHAp was synthesized using microwave-irradiation method and characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), high-resolution scanning electron microscopy-energy-dispersive X-ray spectroscopy (HRSEM-EDX), and transmission electron microscopy (TEM). A total of 75 pre-demineralized coronal dentin specimens were randomly treated with one of the following test agents (n = 15 each): artificial saliva (AS), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), CEnHAp, PHS, and CEnHAp-PHS under pH cycling for 7, 14, and 28 days.
View Article and Find Full Text PDFImproved Enamel Acid Resistance Using Biocompatible Nano-Hydroxyapatite Coating Method.
Materials (Basel)
October 2022
Department of Epidemiology and Public Health, Tokyo Dental College, Tokyo 101-0061, Japan.
In this study, we attempted to develop a dental caries prevention method using a bioapatite (BioHap), an eggshell-derived apatite with nanoparticle size and biocompatibility, with a high-concentration fluoride tooth surface application method. The enamel acid resistance after the application of the proposed method was compared with that of a conventional topical application of fluoride using bovine tooth enamel as an example. The tooth samples were divided into three groups based on the preventive treatment applied, and an acid challenge was performed.
View Article and Find Full Text PDFEggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation.
Int Endod J
January 2022
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
Aim: To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs).
Methodology: Nano-hydroxyapatite was derived from eggshells using a simple combustion method and CMC was prepared from chitosan through a chemical route. Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods.
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