The aberrant expression of microRNAs (miRs) may be involved in tumor growth and progression in human non-small cell lung carcinoma (NSCLC). The present study aimed to investigate the potential roles of miR-191 in NSCLC. Western blotting and reverse transcription-quantitative polymerase chain reaction were performed to assess protein and/or mRNA levels. Scratch wound healing and transwell assays were performed to determine the NSCLC cell migration and invasion. A luciferase demonstrated that CCAAT/enhanced binding protein β (C/EBPβ) was a target of miR-191. Previously, miR-191 has been reported to act as an oncogenic player in multiple human cancers. C/EBPβ has been identified as a target gene of miR-191; however, the roles and underlying mechanisms of miR-191 associated with the regulation of tumor invasion in NSCLC remain unknown. In the present study, it was demonstrated that miR-191 expression levels were higher in human NSCLC tumors compared with in normal adjacent tissue and elevated miR-191 expression levels were closely associated with tumor node metastasis stage in patients with NSCLC. Furthermore, transfection with miR-191 mimic inhibited C/EBPβ expression at the mRNA and protein levels and promoted A549 cell migration and invasion. C/EBPβ was reported to be the direct target gene of miR-191 using a dual luciferase reporter assay. Finally, C/EBPβ siRNA can mimic the effects of miR-191. These findings indicated that miR-191 may function as an oncogene in NSCLC, at least partially due to its negative regulatory on C/EBPβ.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601399 | PMC |
| http://dx.doi.org/10.3892/etm.2019.7668 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of reference microRNAs in skeletal muscle of a canine model of Duchenne muscular dystrophy.
Wellcome Open Res
November 2024
Department of Clinical Science and Services, Comparative Neuromuscular Diseases Laboratory, Royal Veterinary College, London, NW1 0TU, UK.
Article Synopsis
- Duchenne muscular dystrophy (DMD) is a severe condition resulting from mutations in the dystrophin gene, and DE50-MD dogs serve as a model to test new therapies for this disease.
- * Researchers aimed to find stable microRNA (miR) references for normalizing expression data across different ages and muscle groups in both healthy (WT) and DMD-affected dogs.
- * The study identified four stable miRs (miR-191, let-7b, miR-125a, and miR-15a) that can be used to accurately normalize the expression levels of other miRs in muscle, indicating differences in miR levels between DE50-MD and WT dogs.
Clinical Value of MiR-191-5p in Patients with Sepsis and Its Regulatory Role in Sepsis-Related Left Ventricular Systolic Dysfunction.
Int Heart J
December 2024
Department of Critical Care Medicine, Guangzhou Hospital of Integrated Traditional and Western Medicine.
In this study, the expression and clinical value of miR-191-5p in sepsis and sepsis-induced left ventricular systolic dysfunction are explored, and the effects of miR-191-5p on inflammation and cardiac function in rats are examined by establishing a septic rat model.The expression level of serum miR-191-5p was detected via reverse transcription quantitative polymerase chain reaction. To evaluate the diagnostic efficacy of miR-191-5p in sepsis, the receiver operator characteristic curve was established.
View Article and Find Full Text PDFBlood Screening of Femtomole Level Multiple Alzheimer's Disease Biomarkers by Metal Isotopic DNA Walkers.
Anal Chem
December 2024
Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.
Aging is a critical global issue that contributes to the high incidence of Alzheimer's disease (AD). Blood screening emerges as the most promising measure for early diagnosis and intervention of AD due to its noninvasive and low cost. However, the practical application of AD blood screening confronts two significant challenges.
View Article and Find Full Text PDFA multi-class support vector machine classification model based on 14 microRNAs for forensic body fluid identification.
Forensic Sci Int Genet
February 2025
Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China. Electronic address:
MicroRNAs (miRNAs) are promising biomarkers for forensic body fluid identification owing to their small size, stability against degradation, and differential expression patterns. However, the expression of most body fluid-miRNAs is relative (differentially expressed in certain body fluids) rather than absolute (exclusively expressed in a specific body fluid). Moreover, different body fluids contain heterogeneous cell types, complicating their identification.
View Article and Find Full Text PDFClinical Significance of miR-4535 and miR-191-5p in Maternal Serum as Independent Biomarkers for Severe Chorioamnionitis.
Cureus
October 2024
Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, JPN.
Introduction: Chorioamnionitis, a perinatal condition caused by fetal membrane inflammation, results in preterm birth, neonatal sepsis, necrotizing enterocolitis, and brain disease in infants. However, predicting maternal and fetal prognoses is challenging. We aimed to assess the relationship between fetal infection induced by severe chorioamnionitis or morbidity and the expression levels of serum miR-4535, miR-1915-5p, and miR-191-5p levels, which are promising biomarkers for chorioamnionitis, in pregnant women with chorioamnionitis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!