MicroRNA-145 suppresses osteogenic differentiation of human jaw bone marrow mesenchymal stem cells partially via targeting semaphorin 3A.

: Human jaw bone marrow mesenchymal stem cells (h-JBMMSCs) are multipotent progenitor cells with osteogenic differentiation potential. MicroRNAs (miRNAs) have emerged as crucial modulators of osteoblast differentiation. In this study, we focus on the role of and its target protein in osteoblast differentiation of h-JBMMSCs. : h-JBMMSCs were isolated and cultured in osteogenic medium. mimics and inhibitors were used to elevate and inhibit expression, respectively. Osteogenic differentiation was determined by Alkaline phosphatase (ALP) and Alizarin red S (ARS) staining, and osteogenic marker detection using quantitative real-time reverse transcription PCR (qRT-PCR) assay. Bioinformatic analysis and luciferase reporter assay were used to identify the target gene of . : was down-regulated during osteogenesis of h-JBMMSCs. Inhibition of promoted osteogenic differentiation of h-JBMMSCs, revealed by enhanced activity of alkaline phosphatase (ALP), greater mineralisation, and increased expression levels of the osteogenic markers, such as Runt-related transcription factor 2 (RUNX2), Osterix (OSX), ALP and COL1A1. could negatively regulate semaphorin3A (SEMA3A), which acts as a positive regulator of osteogenesis. inhibitor induced osteogenesis could be partially attenuated by SEMA3A siRNA treatment in h-JBMMSCs. : Our data show that directly targets SEMA3A, and also suggest as a suppressor, plays an important role in the osteogenic differentiation of h-JBMMSCs.