AI Article Synopsis
- MOS (Mannanan oligosaccharide) is a supplement for livestock that enhances nutrient absorption and health, but its structure and bioactive forms are not fully understood.
- The study involved producing MOS through enzymatic hydrolysis of pretreated coconut meal using a stable recombinant mannanase enzyme, which was derived from a specific gene.
- Results showed that sonication improved the surface properties of the copra meal, leading to higher MOS yields, and the pentamer form of MOS (M5) notably enhanced tight junction integrity in cultured T84 cells.
Article Abstract
Background: Mannanan oligosaccharide (MOS) is well-known as effective supplement food for livestock to increase their nutrients absorption and health status, but the structure and identification of bioactive MOS remain unclear. In this study, MOS production was accomplished, using enzymatic hydrolysis of pretreated coconut meal substrate with recombinant mannanase.
Methods: The mannanase gene was cloned from AE24, then expressed in BL21. Purified Mannanase exhibit stability over a wide range of pH and temperature from pH 6-8 and 4 °C to 70 °C, respectively. SEM analysis revealed that sonication could change the surface characteristic of copra meal, which gave better MOS yield, compared to untreated substrates. The separation and purification of each MOS were achieved using Biogel-P2 column chromatography. Determination of biological active MOS species was also investigated. T84 cells were cultured and treated with each of the purified MOS species to determine their tight junction enhancing activity.
Results: Scanning electron microscope imaging showed that pretreatment using sonication could disrupt the surface of copra meal better than grinding alone, which can improve the production of MOS. Pentamer of MOS (M5) significantly increased tight junction integration of T84 cells measured with TEER ( < 0.0001).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611449 | PMC |
| http://dx.doi.org/10.7717/peerj.7206 | DOI Listing |
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