AI Article Synopsis
- * The study focused on the TCSs in Kocuria rhizophila DC2201, known for its antibiotic resistance, to understand how these systems contribute to the bacteria's survival and adaptation.
- * A key TCS identified, the HK8700-RR8701 system, is similar to a system found in Bacillus subtilis, and its characterization through various assays may provide new insights into the survival strategies of K. rhizophila and enhance its practical applications.
Article Abstract
Two-component systems (TCSs) are highly conserved in prokaryotes, endowing cells with multiple physiological functions to respond to changes in the ambient environment. The signaling pathway of a typical TCS consists of a sensory histidine kinase and a response regulator. The TCSs of Kocuria rhizophila, which is usually used as a target strain for various antibiotics and other adverse factors, have captured our interest due to their potential roles in bacterial adaptation for survival. Herein, the distribution and putative biological functions of the TCSs of K. rhizophila DC2201 were analyzed by using bioinformatics, and a preliminary TCS regulatory network was constructed. A representative and important TCS (i.e., HK8700-RR8701 system), which is homologous to the LiaS-LiaR system previously discovered in Bacillus subtilis, was identified and characterized through yeast two-hybrid screening and phosphorylation assays. Detailed information of TCSs is expected to offer novel insights into the adaptation mechanism of K. rhizophila and thus boost its application.
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| http://dx.doi.org/10.1007/s10930-019-09853-4 | DOI Listing |
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