AI Article Synopsis
- The study aimed to analyze the genetic diversity and transmission patterns of Mycobacterium tuberculosis (MTB) in Sri Lanka, a region with limited existing research.
- DNA from 89 MTB isolates collected from new pulmonary tuberculosis patients in Kandy was examined using multiple genetic typing methods, revealing that lineage 4 (Euro-American) was the most prevalent.
- The findings indicated a unique population structure of MTB in Kandy, highlighting a concerning increase in local lineage 4 and potentially drug-resistant strains, necessitating ongoing surveillance of these trends.
Article Abstract
Objective: Sri Lanka is a country where the molecular epidemiology of Mycobacterium tuberculosis (MTB) is poorly explored. Therefore, this study was performed to identify circulating lineages/sub-lineages of MTB and their transmission patterns.
Methods: DNA was extracted from 89 isolates of MTB collected during 2012 and 2013 from new pulmonary tuberculosis patients in Kandy, Sri Lanka and analyzed by spoligotyping, large sequence polymorphism (LSP), mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing, and drug resistance-associated gene sequencing.
Results: The predominant lineage was lineage 4 (Euro-American, 45.9%), followed by lineage 1 (Indo-Oceanic, 29.4%), lineage 2 (East-Asian, 23.5%), and lineage 3 (Central-Asian, 1.2%). Among 26 spoligotype patterns, eight were undesignated or new types and seven of these belonged to lineage 4. Undesignated lineage 4/SIT124 (n=2/8) and SIT3234 (n=8/8) clustered together based on 24-locus MIRU-VNTR typing. The dominant sub-lineage was Beijing/SIT1 (n=19), with the isoniazid resistance katG G944C mutation (Ser315Thr) detected in two of them.
Conclusions: The population structure of MTB in Kandy, Sri Lanka was different from that in the South Asian region. The clonal expansion of locally evolved lineage 4/SIT3234 and detection of the pre-multidrug resistant Beijing isolates from new tuberculosis patients is alarming and will require continuous monitoring.
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| http://dx.doi.org/10.1016/j.ijid.2019.07.001 | DOI Listing |
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