An ideal inhalable aerosol sampler for occupational exposure monitoring would have a sampling efficiency that perfectly matches the inhalable particulate matter (IPM) criterion. Two common aerosol samplers in use worldwide are the closed-face cassette (CFC) and the Institute of Occupational Medicine (IOM) sampler. However, the CFC is known to under-sample, with near zero sampling efficiency for particles >30 µm, whereas the IOM, considered by many to be the "gold standard" in inhalable samplers, has been shown to over-sample particles >60 µm. A new sampler in development incorporates characteristics of both the CFC and the IOM. Like the CFC, it would be disposable, have a simple design, and is intended to be oriented at a 45° downward angle. Like the IOM, the new sampler has a 15-mm inlet diameter and incorporates a 25-mm filter cassette with a protruding lip. The IOM is oriented at 0° to the horizontal, so it is hypothesized that orienting the new sampler at ∼45° downward angle will reduce oversampling of larger particles. In comparison, the CFC's inlet diameter is 4 mm; increasing the size of the inlet should allow the new sampler to have an increased efficiency relative to the CFC for all particles. A unique characteristic of the new sampler is the incorporation of a one-piece capsule-style filter that mimics the IOM's cassette but is made of disposable material. Seven different sizes of alumina particles (mean aerodynamic diameters from 4.9-62.4 µm) were tested (total = 124 samples collected). For each test, six samplers were placed on a manikin located inside a wind tunnel operated at 0.2 m/sec. Results indicated that the new sampler improved on the CFC for smaller particles, providing a larger range for which it matches the IPM criterion, up to 44.3 µm. However, the efficiency was significantly lower in comparison to the IPM criterion for particle sizes above 60 µm. Overall, the new sampler showed promise, but additional modifications may help improve sampling efficiency for larger particles.
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http://dx.doi.org/10.1080/15459624.2019.1632463 | DOI Listing |
BMC Cancer
January 2025
Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China.
Objective: Rapid on-site evaluation (ROSE) of respiratory cytology specimens is a critical technique for accurate and timely diagnosis of lung cancer. However, in China, limited familiarity with the Diff-Quik staining method and a shortage of trained cytopathologists hamper utilization of ROSE. Therefore, developing an improved deep learning model to assist clinicians in promptly and accurately evaluating Diff-Quik stained cytology samples during ROSE has important clinical value.
View Article and Find Full Text PDFBMC Genomics
January 2025
Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, No. 3888 Chenhua Road, Songjiang District, Shanghai, 201602, China.
Background: Despite the rapid advancement of high-throughput sequencing, simple sequence repeats (SSRs) remain indispensable molecular markers for various applied and research tasks owing to their cost-effectiveness and ease of use. However, existing SSR markers cannot meet the growing demand for research on lotus (Nelumbo Adans.) given their scarcity and weak connections to the lotus genome.
View Article and Find Full Text PDFActa Parasitol
January 2025
Department of Veterinary Medicine, Federal University of Paraná, Rua Dos Funcionários, 1540, Curitiba, Paraná, 80035-050, Brazil.
Purpose: The aim of the present study was to establish a SYBR Green-based real-time PCR assay for detection of the Nc5 segment from the Neospora caninum genome.
Methods: The oligonucleotides sequences targeting the Nc5 gene previously reported and designed in-house were validated. Two Primer sets were evaluated and tested in four different combinations.
Sci Rep
January 2025
Department of Signal Theory and Communications, Universidad de Valladolid, 47002, Valladolid, Spain.
Sound-based uroflowmetry (SU) offers a non-invasive alternative to traditional uroflowmetry (UF) for evaluating lower urinary tract dysfunctions, enabling home-based testing and reducing the need for clinic visits. This study compares SU and UF in estimating urine flow rate and voided volume in 50 male volunteers (aged 18-60), with UF results from a Minze uroflowmeter as the reference standard. Audio signals recorded during voiding were segmented and machine learning algorithms (gradient boosting, random forest, and support vector machine) estimated flow parameters from three devices: Ultramic384k, Mi A1 smartphone, and Oppo smartwatch.
View Article and Find Full Text PDFCurr Microbiol
January 2025
Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Bovine mastitis is the most widespread disease that causes financial loss in the dairy industry. Staphylococcus aureus is a well-researched multidrug-resistant opportunistic bacterium that is frequently linked to subclinical mastitis and causes significant economic losses. A further problem in the management of S.
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