A novel antimicrobial peptide named NP-6 was identified in our previous work. Here, the mechanisms of the peptide against Escherichia coli (E. coli) were further investigated, as well as the peptide's resistance to temperature, pH, salinity, and enzymes. The transmission electron microscopy (TEM), confocal laser scanning microcopy (CLSM), and flow cytometric (FCM) analysis, combined with measurement of released K, were performed to evaluate the effect of NP-6 E. coli cell membrane. The influence of NP-6 on bacterial DNA/RNA and enzyme was also investigated. The leakage of K demonstrated that NP-6 could increase the permeability of E. coli cell membrane. The ATP leakage, FCM, and CLSM assays suggested that NP-6 caused the disintegration of bacterial cell membrane. The TEM observation indicated that NP-6 could cause the formation of empty cells and debris. Besides, the DNA-binding assay indicated that NP-6 could bind with bacterial genomic DNA in a way that ethidium bromide (EB) did, and suppress the migration of DNA/RNA in gel retardation. Additionally, NP-6 could also affect the activity of β-galactosidase. Finally, the effect of different surroundings such as heating, pH, ions, and protease on the antimicrobial activity of NP-6 against E. coli was also investigated. Results showed that the peptide was heat stable in the range of 60~100 °C and performed well at pH 6.0~8.0. However, the antimicrobial activity of NP-6 decreased significantly in the presence of Mg/Ca, and after incubation with trypsin/proteinase K. The results will provide a theoretical support in the further application of NP-6.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00253-019-09981-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background: Modern studies suggest that lifestyle changes of the indigenous Arctic residents lead to the loss of the "adaptive polar type of metabolism", which is characterized by the intensification of protein metabolism, optimization of lipid metabolism, and minimization of carbohydrate metabolism at low insulin concentrations. How to survive the era of change?
Aim: To assess insulinemia, glycemia, β-cell secretory activity, and insulin sensitivity in Arctic indigenous people in relation to their lifestyle.
Materials And Methods: A cross-sectional study of a population of indigenous Arctic residents (Nenets, Komi) aged from 22 to 60 years was conducted.
The Impact of Orthodontic Adhesive Containing Resveratrol, Silver, and Zinc Oxide Nanoparticles on Shear Bond Strength: An In Vitro Study.
Cureus
August 2024
Orthodontics and Dentofacial Orthopedics, School of Dental Sciences, Krishna Vishwa Vidyapeeth (Deemed to be University), Karad, IND.
Introduction The goal of orthodontic treatment is to provide patients with esthetic smiles and functional occlusion. Despite best efforts and continuous evolution of materials, white spot lesions present a persistent hindrance to the desired treatment outcome. Nanoparticles have shown efficacy in reducing microbial activity; however, currently, there is a need for natural anti-cariogenic compounds with minimal side effects.
View Article and Find Full Text PDFBiobanking, digital health and privacy: the choices of 1410 volunteers and neurological patients regarding limitations on use of data and biological samples, return of results and sharing.
BMC Med Ethics
September 2024
Clinical Neurochemistry Unit and Biobank, IRCCS Santa Lucia Foundation, via Ardeatina 354, Rome, Italy.
Article Synopsis
- The text discusses the ethical and legal considerations surrounding the collection of biological samples and data for research, especially in the context of neurological diseases and the role of biobanking.
- A study evaluated attitudes towards donating samples for biobanks among 1,454 participants, including healthy volunteers and patients with neurological conditions, with a high level of agreement on participation in biobanking.
- Key findings show significant differences in preferences regarding the return of results and data sharing, notably that healthy volunteers were more likely to want to remain uninformed about results compared to patients.
Antimicrobial resistance in intensive care patients hospitalized with SEPSIS: a comparison between the COVID-19 pandemic and pre-pandemic era.
Front Med (Lausanne)
May 2024
Department of Anesthesiology, Critical Care Medicine and Emergency, Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University Chieti-Pescara, Chieti, Italy.
Article Synopsis
- COVID-19, caused by the SARS-CoV-2 virus, has led to millions of deaths and significant lifestyle changes, particularly impacting patients with sepsis in ICUs, where septic complications have a high mortality rate.
- A clinical study was conducted at SS Annunziata Hospital in Italy, analyzing ICU patients diagnosed with sepsis from 2018 to 2021, dividing them into groups based on the pandemic and COVID-19 status.
- Out of 1,559 ICU admissions, 211 patients met the sepsis criteria, revealing high mortality rates, especially amongst COVID-19 patients, with a notable relationship between mortality and scores on APACHE III and SOFA assessments.
Pinworm Blatticola blattae infection and survival of starving German cockroach Blattella germanica.
Parasitol Res
April 2024
Laboratory of Parasitology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa, 252-5201, Japan.
The German cockroach Blattella germanica is commonly infected with the pinworm Blatticola blattae. To determine the effect of pinworm infection on cockroach survival, we artificially altered the pinworm infection status of cockroaches and determined the number of survival days under no-feeding conditions, with and without opportunities for fecal feeding. Four lines of the German cockroach (Wn, Wp, Nn, and Np groups) were used in the experiment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!