Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis of a Namibian strain of showed the presence of a number of yessotoxins (YTXs). Principal among these were YTX (), homoYTX (), and a tentative hydroxylated analogue that did not correspond to any previously confirmed YTX structures. Culturing the strain afforded sufficient biomass for purification of the new analogue through a series of solvent partitioning and chromatographic steps, yielding ∼0.9 mg as a solid. NMR spectroscopy, ion-trap mass spectrometry, and HRMS identified the new analogue as 24-hydroxyYTX (). Purified 24-hydroxyYTX was quantitated by NMR, and its relative toxicity evaluated using two embryonic zebrafish toxicity assays. 24-HydroxyYTX demonstrated reduced toxicity compared to YTX.
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