Chemical constituents and biological activities of the fruits of de Wilde.

The -hexane extract of fruits (Myristicaceae family), exhibiting strong anti-acetylcholinesterase activity, was investigated by gas chromatography/mass spectrometry and then purified by column chromatography. Guided by GC/MS profiling and bioassay, chromatographic separations led to the isolation of five new compounds: two anacardic acid derivatives -, two cardanol derivatives - and a cardol derivative , along with mixtures of known phenolic lipids -. The chemical structures were determined by various spectroscopic methods. New isolated compounds - were evaluated for their cytotoxicity and anti-acetylcholinesterase activity. Cardanol and cardol were the most active compounds in the acetylcholinesterase inhibitory assay with IC values of 2.60 ± 0.24 µM and 2.46 ± 0.23 µM, respectively. Cardanol and cardol showed moderate cytotoxicity against Hela and MCF-7 cancer cell lines with IC values ranging from 31.36 ± 0.41 µM to 41.30 ± 2.49 µM.