Rapamycin exposure to host and to adult worms affects life history traits of Heligmosomoides bakeri.

Parasite life history can be affected by conditions of the host and of the external environment. Rapamycin, a known immunosuppressant of mammals, was fed to laboratory mice that were then infected with the Trichostrongylid nematode Heligmosomoides bakeri to determine if host rapamycin exposure would affect parasite survival, growth, and reproduction. In addition, adult worms from control fed mice were directly exposed to rapamycin to assess if rapamycin would affect worm viability and ex vivo reproduction. We found that host ingestion of rapamycin did not affect H. bakeri survival or growth for male or female worms, but female worms had increased reproduction both in vivo and when removed from the host and cultured ex vivo. After direct rapamycin exposure, motility of female worms was greater at low levels of rapamycin compared to high levels of rapamycin or high levels of DMSO (the vehicle used to solubilize rapamycin) in control media, but was similar to females in low levels of DMSO in control media. Male motility was not affected by the presence of rapamycin or DMSO in the media. Ex vivo egg deposition was higher when exposed to rapamycin than when cultured in control media that contained DMSO, regardless of DMSO dose. Overall, we conclude that host ingestion of rapamycin or direct exposure to rapamycin was generally favorable or neutral for parasite life history traits.