The herbicide atrazine is heavily applied in the U.S. Midwest to control broadleaf weeds. It enters local streams and rivers through runoff and seepage, and exposure can affect non-target aquatic organisms, like crayfish. We examined sublethal effects of atrazine on the expression and activity of the detoxification enzymes cytochrome P450 (CYP450) and glutathione-S-transferase (GST) in crayfish. Crayfish were exposed to 0, 10, 40, 80, 100 and 300 ppb atrazine for 1, 2, 4, 7 and 10 days. Their hepatopancreas was collected and CYP450 expression and GST activity was analyzed. Atrazine exposure caused differential expression and activity of CYP450 and GST. CYP450 expression varied over exposure concentrations and time. Further, GST activity significantly increased following a 2 day, 10 ppb exposure to atrazine and a 300 ppb atrazine exposure for all days tested. We found that atrazine detoxification is a dynamic process that changes with the length and intensity of atrazine exposure.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00128-019-02674-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Environmentally-Relevant Concentrations of Atrazine Had Minor Impacts on Gut Microbiota and Liver Metabolite in Juvenile Turtles.
Arch Environ Contam Toxicol
January 2025
Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, Zhejiang, China.
Toxic effects of herbicide atrazine (ATR) have been evaluated in various aquatic organisms, but our understanding of its potential impacts in reptile species remains limited. In this study, the functional performances, and gut microbiota and liver metabolite alterations of ATR-exposed Mauremys sinensis juveniles were measured to evaluate its potential toxic effects in turtles. ATR exposure had no impact on the growth rate, but would allow turtles to right themselves more quickly.
View Article and Find Full Text PDFHigh-throughput screening to identify endocrine disruptors: Contribution of low-resolution tandem MS and high-resolution MS.
Anal Chim Acta
February 2025
Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000, Rennes, France.
Background: Considering the large diversity of chemicals present in the environment and the need to study their effects (alone or as mixtures), the development of high-throughput in vitro assays in line with the Replacement, Reduction, Refinement (3R) strategy is essential for chemical risk assessments.
Results: We developed a robust analytical workflow based on both low resolution tandem mass spectrometry (MS/MS) and high-resolution mass spectrometry (HRMS) to quantify 13 steroids in NCI-H295R cell culture medium, human plasma and serum. The workflow was validated by screening media from the NCI-H295R cell line exposed in dose-response experiments to 5 endocrine disruptors (EDs) such as bisphenol A, prochloraz, ketoconazole, atrazine and forskolin.
Interspecific differences in the response of autotrophic microorganisms to atrazine and S-metolachlor exposure.
Ecotoxicol Environ Saf
January 2025
UR EABX, Inrae, Cestas, France. Electronic address:
Atrazine and S-metolachlor are herbicides widely used on corn and soybean crops where they are sometimes found in concentrations of concern in nearby aquatic ecosystems, potentially affecting autotrophic organisms. The aim of this study was to investigate the response of the green algae Enallax costatus, the diatom Gomphonema parvulum and a culture of the cyanobacteria Phormidium sp. and Microcystis aeruginosa, to atrazine and S-metolachlor alone and in mixture (0, 10, 100 and 1000 µg.
View Article and Find Full Text PDFTransgenerational Effects of Atrazine on Daphnia magna Based on Life-History Traits and Population Dynamics.
Bull Environ Contam Toxicol
December 2024
Jiangsu Key Laboratory for Bioaffiliationersity and Biotechnology, School of Biological Sciences, Nanjing Normal University, Nanjing, 210023, China.
Atrazine is a predominant herbicide globally, and its residues are commonly found in natural water bodies due to its extensive use. Atrazine is known for its detrimental effects on the reproductive abilities of aquatic plants and animals. Our study explored the impact of maternal exposure to atrazine on the survival and performance of offspring using the water flea Daphnia magna as a model organism.
View Article and Find Full Text PDFMorphological and cellular effects in Boana faber tadpoles (Anura: Hylidae) exposed to atrazine-based herbicide and glyphosate-based herbicide and their mixtures.
Environ Sci Pollut Res Int
January 2025
Laboratório de Ecologia e Conservação, Universidade Federal da Fronteira Sul, Campus Erechim, RS 135 - km 72, nº 200, Erechim, RS, Brazil.
Atrazine and glyphosate are considered some of the main pollutants for aquatic ecosystems, directly and indirectly affecting non-target organisms, such as amphibians. This study aimed to evaluate the sublethal effects of different concentrations of atrazine-based herbicide (ABH) and glyphosate-based herbicide (GBH) commercial formulations, both individually and in a mixture, through toxicity tests on the larval stage of Boana faber. Tadpoles were exposed to concentrations of ABH (2, 9.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!