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From dual nucleic acid co-extraction to co-sequencing: A highly integrated next-generation forensic DNA and RNA sequencing experimental workflow.
Forensic Sci Int Genet
January 2025
Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; School of Forensic Medicine, Kunming Medical University, Kunming 650500, China. Electronic address:
DNA and RNA markers are significant in forensic practices, such as individual and body fluid identification. However, forensic DNA and RNA markers were separately analyzed in most forensic experiments, which resulted in large amounts of sample consumption, complex procedures, and weak inter-evidence correlation. While several integrated methods based on capillary electrophoresis and next-generation sequencing technologies were reported, integrated procedures were mostly on nucleic acid co-extraction, co-electrophoresis, or co-sequencing, and the number and type of markers co-tested were limited.
View Article and Find Full Text PDFCharacterization of Optokinetic Nystagmus in Healthy Participants With a Novel Oculography Device.
Otolaryngol Head Neck Surg
January 2025
Department of Otolaryngology-Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, USA.
Objective: To develop a proof-of-concept smart-phone-based eye-tracking algorithm to assess non-pathologic optokinetic (OKN) nystagmus in healthy participants. Current videonystagmography (VNG) is typically restricted to in-office use, and advances in portable vestibular diagnostics would yield immense public health benefits.
Study Design: Prospective cohort study.
Validation of UniverSeg for Interventional Abdominal Angiographic Segmentation.
J Imaging Inform Med
January 2025
Department of Radiation Oncology, Henry Ford Health, Detroit, MI, USA.
Automatic segmentation of angiographic structures can aid in assessing vascular disease. While recent deep learning models promise automation, they lack validation on interventional angiographic data. This study investigates the feasibility of angiographic segmentation using in-context learning with the UniverSeg model, which is a cross-learning segmentation model that lacks inherent angiographic training.
View Article and Find Full Text PDFPostoperative Lung Function Advantages in Pulmonary Segmentectomy for Early-Stage Lung Cancer.
Thorac Cancer
January 2025
Department of Thoracic Surgery, University and Hospital Trust-Ospedale Borgo Trento, Verona, Italy.
In early-stage lung cancer, lung function appears to be less compromised after segmentectomy than lobectomy, though the advantage seems modest. We aimed to re-assess postoperative lung function in surgical patients, with a particular focus on the diffusion capacity for carbon monoxide (DL). We evaluated all patients who underwent either lobectomy or segmentectomy for T1a-c lung cancer at our center between March 2016 and March 2023.
View Article and Find Full Text PDFThe feasibility of assessing prognosis over 3 years in persons with a previous stroke/transient ischemic attack in general practice.
Pilot Feasibility Stud
January 2025
School of Medicine, University of Limerick, Limerick, Ireland.
Background: Stroke has devastating consequences for survivors. Hypertension is the most important modifiable risk factor, and its management largely takes place in primary care. However, most stroke-based research does not occur in this setting.
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